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  4. FXR1 Recombinant Monoclonal Antibody

FXR1 Recombinant Monoclonal Antibody

  • 中文名稱：
    FXR1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA162896A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: K562 whole cell lysate(30μg)
      All lanes: FXR1 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 70 kDa
      Observed band size: 70-85 kDa
      Exposure time：1min
    • Immunofluorescence staining of Hela cell with CSB-RA162896A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing A431 cells stained with CSB-RA162896A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    Fragile X mental retardation syndrome-related protein 1 (hFXR1p), FXR1
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human FXR1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    9H11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2000
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs.
  • 基因功能參考文獻：
    1. in head and neck squamous cell carcinoma, fragile X mental retardation syndrome related protein 1 (Fxr1) overexpression correlates with reduced F-box protein 4 (Fbxo4) levels. PMID: 29142209
    2. Inhibition of the remaining family member FXR1 selectively blocks cell proliferation in human cancer cells containing homozygous deletion of both TP53 and FXR2 in a collateral lethality manner. PMID: 28767039
    3. Our findings support our a priori hypothesis of a possible interaction between GSK3B (rs12630592 T allele) and FXR1 (rs496250 A allele) in mood disorder patients. PMID: 28242499
    4. human microbiota was able to reduce the levels of tauro-beta-muricholic acid and induce expression of FXR target genes Fgf15 and Shp in ileum after long-term colonization. We show that a human microbiota can change BA composition and induce FXR signaling in colonized mice, but the levels of secondary BAs produced are lower than in mice colonized with a mouse microbiota PMID: 27956475
    5. These data reveal a new role of FXR1 in controlling induction of monocyte migration. PMID: 27229378
    6. FXR1 binds and destabilizes p21 mRNA, binds and stabilizes TERC RNA and suppresses the cellular senescence in oral cancer cells. PMID: 27606879
    7. FXR1P interacts with CMAS, and that FXR1P may enhance the activation of sialic acid via interaction with CMAS, and increase GM1 levels to affect the development of the nervous system, thus providing evidence for further research into the pathogenesis of FXS. PMID: 27357083
    8. P97 interacts with 3' UTR-binding FXR1a-associated microRNPs and with PARN, which binds mRNA 5' caps, forming a specialized complex to translate recruited mRNAs in these altered canonical translation conditions. PMID: 26942679
    9. An accumulation of 8 SNPs in the fragile gene family (FMR1, FXR1 and FXR2)were found associated with autistic traits in a sample of male patients. PMID: 26612855
    10. FXR1P is a GSK3beta substrate with a role in regulating mood and emotion processing PMID: 26240334
    11. The mechanisms by which FXR1 executes its regulatory function by forming a novel complex with two other oncogenes, protein kinase C, iota and epithelial cell transforming 2, located in the same amplicon via distinct binding mechanisms, were identified. PMID: 25733852
    12. binding of PKP1/3 to FXR1 was RNA independent, and both PKP3 and FXR1 stabilized PKP2 mRNA. PMID: 25225333
    13. Deregulation of Fragile X-related protein 1 by the lipodystrophic lamin A p.R482W mutation elicits a myogenic gene expression program in preadipocytes. PMID: 24108105
    14. findings showed that FXR1P interacts with BTF in vivo and proved that FXR1P and BTF can co-localize mainly in the cytoplasm around the nucleus PMID: 24389646
    15. FXR1 and UPF1 may have a functional role in prostate cancer progression and metastasis. PMID: 23881279
    16. Our study describes a novel role of FXR1P that has crucial implications for the understanding of its role during myogenesis and muscle development PMID: 23555284
    17. Data show that the nuclear localization signals of the FXR1 and FXR2 comprise tandem Tudor domain architectures. PMID: 21072162
    18. These results indicate that FMR1 gene function is evolutionarily conserved in neural mechanisms and cannot be compensated by either FXR1 or FXR2, but that all three proteins can substitute for each other in non-neuronal requirements. PMID: 20442204
    19. Fxr1p shows a more tissue-specific expression particularly during late embryonic development. PMID: 15968590
    20. Results show that three genes, namely FXR1, CLAPM1 and EIF4G, are most frequently overexpressed in the center of the amplified domain in squamous cell carcinomas. PMID: 17290396
    21. A crosslinking-coupled affinity purification method was used to isolate TNF-alpha AU-rich element-associated proteins: two microRNP-related proteins, FXR1 and AGO2 were found that associate during translation activation. PMID: 17382880
    22. FXR1 mRNA splicing pattern is altered in facioscapulohumeral muscular dystrophy myoblasts. PMID: 18628314
    23. FXR1P and FXR2P KH2 domains bind G-quadruplex and kissing complex RNA with the same affinity as the FMRP KH2 domain. PMID: 19487368

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  • 亞細胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    FMR1 family
  • 組織特異性：
    Expressed in all tissues examined including heart, brain, kidney and testis.
  • 數據庫鏈接：

    HGNC: 4023

    OMIM: 600819

    KEGG: hsa:8087

    STRING: 9606.ENSP00000350170

    UniGene: Hs.478407