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  4. USP13 Recombinant Monoclonal Antibody

USP13 Recombinant Monoclonal Antibody

  • 中文名稱：
    USP13 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA194330A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of ubiquitin specific peptidase 13 expression in HepG2 cells using ubiquitin specific peptidase 13 antibody. Green, isotype control; red, ubiquitin specific peptidase 13.
    • Immunocytochemical staining of HepG2 cells with Ubiquitin specific peptidase 13?antibody. Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 13?was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using ubiquitin specific peptidase 13 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ubiquitin specific peptidase 13 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    USP13; Ubiquitin Specific Peptidase 13; Ubiquitin Specific Protease 13 (Isopeptidase T-3); Ubiquitin-Specific-Processing Protease 13; Ubiquitin Carboxyl-Terminal Hydrolase 13; Deubiquitinating Enzyme 13; Ubiquitin Thioesterase 13; Isopeptidase T-3; IsoT-3; ISOT-3; ISOT3; Ubiquitin Specific Peptidase 13 (Isopeptidase T-3); Ubiquitin Thiolesterase 13; EC 3.4.19.12; EC 3.1.2.15
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human USP13 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    13H7
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation (ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation (ERAD). Also regulates ERAD through the deubiquitination of UBL4A a component of the BAG6/BAT3 complex. Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro; however, additional experiments are required to confirm such data.
  • 基因功能參考文獻：
    1. USP13 is a regulator of DNA repair.USP13 regulates RAP80-BRCA1 complex foci formation.The role of USP13 in the neoplasm drug resistance. PMID: 28569838
    2. These results reveal an important metabolism-centric role of USP13, which may lead to potential therapeutics targeting USP13 in ovarian cancers. PMID: 27892457
    3. in the samples of 293T cell lines after the overexpression of USP13 and USP13 C345S, vinculin exhibited an increased expression, suggesting that it may be a candidate substrate of USP13. PMID: 28498477
    4. Deubiquitinating enzyme 13 (USP13) regulates myeloid cell leukemia sequence 1 protein (MCL1) stability in lung and ovarian cancer cells. PMID: 29335437
    5. our results demonstrate that AR can promote melanoma metastasis via altering the miRNA-539-3p/USP13/MITF/AXL signal and targeting this newly identified signal with AR degradation enhancer ASC-J9 may help us to better suppress the melanoma metastasis. PMID: 27869170
    6. USP13 and FBXL14 play opposing roles in the regulation of glioma stem cells (GSCs) through reversible ubiquitination of c-Myc. PMID: 27923907
    7. Down-regulation of USP13 mediates PTEN protein loss and fibroblast phenotypic change, and thereby plays a crucial role in idiopathic pulmonary fibrosis pathogenesis. PMID: 26453058
    8. USP13 is a tumour-suppressing protein that functions through deubiquitylation and stabilization of PTEN. PMID: 24270891
    9. to our knowledge, USP13 is the first deubiquitinating enzymes identified to modulate STAT1 and play a role in the antiviral activity of IFN against DEN-2 replication. PMID: 23940278
    10. USP10 mediates the deubiquitination of p53, regulating deubiquitination activity of USP10 and USP13 by Beclin1 provides a mechanism for Beclin1 to control the levels of p53. PMID: 21962518
    11. Through deubiquitination, USP13 stabilizes and upregulates MITF protein levels. PMID: 21811243
    12. study identifies a new layer of Siah2 regulation mediated by USP13 binding to ubiquitinated Siah2 protein with a concomitant inhibitory effect on its activity under normoxia PMID: 21659512

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  • 蛋白家族：
    Peptidase C19 family
  • 組織特異性：
    Highly expressed in ovary and testes.
  • 數據庫鏈接：

    HGNC: 12611

    OMIM: 603591

    KEGG: hsa:8975

    STRING: 9606.ENSP00000263966

    UniGene: Hs.175322