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  4. RAD21 Recombinant Monoclonal Antibody

RAD21 Recombinant Monoclonal Antibody

  • 中文名稱：
    RAD21 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA982110A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of RAD21 expression in HepG2 cells using RAD21 antibody. Green, isotype control; red, RAD21.
    • Western blotting analysis using RAD21 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD21 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    RAD21; RAD21 Cohesin Complex Component; HHR21; SCC1; KIAA0078; Double-Strand-Break Repair Protein Rad21 Homolog; Sister Chromatid Cohesion 1; Nuclear Matrix Protein 1; SCC1 Homolog; Kleisin; NXP-1; HR21; NXP1; Protein Involved In DNA Double-Strand Break Repair; RAD21 (S. Pombe) Homolog; RAD21 Homolog (S. Pombe); RAD21 Homolog; HRAD21; CDLS4; MCD1; MGS
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human RAD21 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21A1
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

  • 功能：
    As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter. May play a role in embryonic gut development, possibly through the regulation of enteric neuron development.; May promote apoptosis.
  • 基因功能參考文獻：
    1. evidence for a possible pathogenetic role of RAD21 promoter methylation in the development of chronic lymphocytic leukemia. PMID: 29587287
    2. With tissue microarrays of hepatocellular carcinoma (HCC) patients, we determined the prognostic values of the core genes in the network and found that RAD21, CDK1, and HDAC2 expression levels were negatively associated with overall survival for HCC patients. PMID: 28434945
    3. This study together with previous reports suggests incomplete penetrance associated with RAD21 variants and these individuals may therefore be underdiagnosed. PMID: 27882533
    4. Data indicate that RAD21 plays an important role in cellular senescence breast cancer cells, mainly through RB1 pathway activation via c-Myc downregulation. PMID: 26529363
    5. Reduced RAD21 destabilizes high-level gene amplification by disrupting pre-replication complex bindings in human cancers with chromosomal instability. PMID: 26420833
    6. found well-positioned CTCF and RAD21 peaks approximately 60-80 bp upstream of the TSS in the unidirectional genes. The peak heights were related to the amount of antisense transcription. Results provide insights into the distribution of histone modifications at promoters and suggest a novel role of CTCF and cohesin as regulators of transcriptional direction. PMID: 25881024
    7. Studied the role of looping in large-scale (supra Mb) folding of human chromosomes by knocking down the gene that codes for CTCF and the one coding for Rad21, an essential subunit of cohesin. PMID: 25299688
    8. Elevated RAD21 expression tracks with reactivation of L1 expression in human sporadic colorectal cancer. PMID: 25464844
    9. Some patients with chronic intestinal pseudo-obstruction carry mutations in RAD21 that disrupt the ability of its product to regulate genes such as RUNX1 and APOB. PMID: 25575569
    10. CTCF and Rad21 act as host cell restriction factors for Kaposi's sarcoma-associated herpesvirus (KSHV) lytic replication by modulating viral gene transcription. PMID: 24415941
    11. cohesin's proposed DNA exit gate is formed by interactions between Scc1 and the coiled-coil region of Smc3. PMID: 25414306
    12. Cross-sectional deep-sequencing analysis for clonal hierarchy demonstrated STAG2, SMC3, and RAD21 mutations to be ancestral in 18%, 18%, and 47% of cases, respectively, and each expanded to clonal dominance concordant with disease transformation PMID: 25006131
    13. RAD21 expression in CRC is associated with aggressive disease especially in KRAS mutant tumours and resistance to chemoradiotherapy. PMID: 24548858
    14. This study reports the first intragenic deletion and frameshift mutations identified in RAD21 in two patients presenting with atypical Cornelia de Lange syndrome. PMID: 24378232
    15. -RAD21 and EIF3H, both on chromosome 8q23, CHRAC1 on chromosome 8q24.3 and TANC2 on chromosome 17q23-were confirmed to be driver genes regulating the proliferation/survival of clonogenic breast cancer cells PMID: 24148822
    16. RAD21 expression confers poor prognosis and resistance to chemotherapy in high grade luminal, basal and HER2 breast cancers. PMID: 21255398
    17. Rad21 binds to SA proteins through two SA-binding motifs on Rad21. PMID: 23874961
    18. ASURA specifically binds to chromatin when Scc1 is associated with chromatin. PMID: 23548868
    19. RAD21 expression is associated with a poorer prognosis in BRCA2, but not in BRCA1 breast cancers. PMID: 22537934
    20. show that the Smc5/6 subunit Mms21 sumoylates multiple lysines of the cohesin subunit Scc1 PMID: 22751501
    21. RAD21 mutations cause a human cohesinopathy PMID: 22633399
    22. Rad21 cleavage by calpain-1 promotes separation of chromosome arms, which coincides with a calcium-induced partial loss of cohesin at several chromosomal loci. PMID: 21876002
    23. hRAD21 was significantly differently expressed in cells maintaining telomere length through a telomerase-independent mechanism, termed the alternative lengthening of telomeres (ALT). PMID: 20364118
    24. Rad21 protein depletion causes centrosome defects in interphase and prophase of mitosis. PMID: 20404533
    25. Rad21 protein are cleaved by separase are required for the localization of Rad21 to the centrosome. PMID: 20404544
    26. As compared to the telomerase group, the alternative lengthening of telomeres group presents higher expression of hRad21, thinner tumor invasion depth, and higher survival rate. PMID: 18197499
    27. Caspase proteolysis of the cohesin component RAD21 promotes apoptosis PMID: 11875078
    28. RAD21 subunit of the cohesin complex directly interacts with ATPase subunit SNF2; mapping of RAD21, SNF2 and Mi2 binding sites reveals specific association of these three proteins with DNA elements containing Alu sequences PMID: 12198550
    29. Scc1 is essential for the association of kinetochores with microtubules. PMID: 12200439
    30. plays a role in apoptosis, and its cleavage during apoptosis may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway PMID: 12417729
    31. RAD21 shows increased expression in clinical prostate carcinomas and is also amplified in 30-40% of xenografts and hormone-refractory tumors. PMID: 14603436
    32. Phosphorylation of SA2 is essential for cohesin dissociation during prophase and prometaphase, but is not required for cohesin cleavage by separase. PMID: 15737063
    33. RAD21 suppression enhances the antitumor activity of chemotherapeutic agents acting via induction of DNA damage PMID: 15767545
    34. The RAD21 gene was closely related to the invasion and metastasis of cancer cells. PMID: 16416296
    35. These data argue that the regulation of the IL-3 and the GM-CSF promoters depends on the positions of their enhancers relative to the conserved CTCF/cohesin-binding sites. PMID: 19158269
    36. The depletion of either CTCF or RAD21 disrupts the chromatin loop structure, together with significant changes in the APO expression. PMID: 19322193
    37. Ectopic expression of Rad21 repressed CTCF-regulated transcription of KSHV lytic genes. PMID: 19369356

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  • 相關疾病：
    Cornelia de Lange syndrome 4 (CDLS4)
  • 亞細胞定位：
    [Double-strand-break repair protein rad21 homolog]: Nucleus. Nucleus matrix. Chromosome. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole.; [64-kDa C-terminal product]: Cytoplasm, cytosol. Nucleus.
  • 蛋白家族：
    Rad21 family
  • 組織特異性：
    Expressed in the gut (at protein level).
  • 數(shù)據(jù)庫鏈接：

    HGNC: 9811

    OMIM: 606462

    KEGG: hsa:5885

    STRING: 9606.ENSP00000297338

    UniGene: Hs.81848