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  4. PIK3R3 Recombinant Monoclonal Antibody

PIK3R3 Recombinant Monoclonal Antibody

  • 中文名稱：
    PIK3R3 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA791880A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      , Positive WB detected in:Mouse brain tissue lysate(30μg), Rat brain lysate(30μg)
      All lanes: PI3 kinase p55 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 54 kDa
      Observed band size: 54 kDa
      Exposure time：120s
    • IHC image of CSB-RA791880A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing MCF-7 cells stained with CSB-RA791880A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    DKFZp686P05226 antibody; FLJ41892 antibody; OTTHUMP00000009783 antibody; OTTHUMP00000009786 antibody; p55 antibody; p55 gamma antibody; P55G_HUMAN antibody; p55PIK antibody; Phosphatidylinositol 3 kinase regulatory subunit gamma antibody; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 3 antibody; Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma antibody; Phosphatidylinositol 3-kinase regulatory subunit gamma antibody; Phosphoinositide 3 kinase regulatory subunit 3 (gamma) antibody; Phosphoinositide 3 kinase regulatory subunit 3 antibody; Phosphoinositide 3 kinase regulatory subunit polypeptide 3 antibody; Phosphoinositide 3 kinase, regulatory subunit 3 (p55, gamma) antibody; Phosphoinositide 3 kinase, regulatory subunit, polypeptide 3 (p55, gamma) antibody; PI3 kinase p85 subunit gamma antibody; PI3-kinase regulatory subunit gamma antibody; PI3-kinase subunit p55-gamma antibody; PI3K regulatory subunit gamma antibody; Pik3r3 antibody; PtdIns 3 kinase p85 gamma antibody; PtdIns-3-kinase regulatory subunit gamma antibody; PtdIns-3-kinase regulatory subunit p55-gamma antibody
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    A synthesized peptide from human PIK3R3 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    2E2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.
  • 基因功能參考文獻：
    1. Results showed that PIK3R3 was upregulated in colorectal cancer (CRC) tissues and inversely correlated with miR-411 expression. Its expression is regulated by miR-411 which targets its 3'UTR. PMID: 29207180
    2. PIK3R3 could promote the metastasis of Pancreatic Cancer by facilitating ZEB1 induced Epithelial-Mesenchymal Transition. PMID: 29719293
    3. Using case-control study and functional analyses, study have identified SNP rs7536272 A > G, located in the promoter region of PIK3R3, acts as a risk factor of GC through enhancing the transcriptional activity of PIK3R3, contributing to increased expression of PIK3R3. PMID: 29802999
    4. The findings suggest that miR-212 acts as a tumor suppressor in colorectal cancer by directly targeting PIK3R3. PMID: 28944887
    5. miR-181a2/181b2 prominently dampened cell-cycle progression, suppressed cell growth, and promoted apoptosis of tumor cells in vitro They also effectively impeded tumor formation and growth in vivo miR-181a2/181b2 exert the tumor suppressor ability by depressing the direct target PIK3R3 (p55gamma) and consequently modulating the PIK3R3/Akt/FoxO signaling pathway PMID: 27503199
    6. p55PIK significantly stimulated the expression of AFP by activating NF-kappaB signaling pathway in hepatocellular carcinoma cells. PMID: 28970114
    7. Study showed that PIK3R3 was a target of miR-365 and its expression was upregulated and inversely correlated with miR-365 expression in glioma tissues. PMID: 28260020
    8. PIK3R3 is a direct and functional downstream target of miR-152. PIK3R3 expression was upregulated and was inversely correlated with miR-152 expression in clinical CRC tissues. Downregulation of PIK3R3 mimicked the tumor-suppressive effects of miR-152 overexpression in CRC cells. PMID: 26820128
    9. p55PIK-PI3K signaling can contribute to imatinib resistance in gastrointestinal stromal tumors by increasing KIT expression. PMID: 26587973
    10. The proliferation, migration and invasion was decreased in ovarian SKOV3 when HOTAIR or PIK3R3 was silenced. PMID: 26826873
    11. PIK3R3 was identified as a crucial target gene of miR-132. PMID: 26252738
    12. our findings provide new insights into tumor suppression by miR-511 by negatively regulating the PIK3R3/AKT/mTOR signaling pathway PMID: 25608840
    13. p55PIK could be a substrate of activated caspase 6 during paraquat-induced apoptosis, leading to loss of original biological functions and redistribution to disturb cell cycle progression. PMID: 24130211
    14. TGF-beta/NKX2.1/PIK3R3 axis is crucial in the TGF-beta-induced inhibition of cell proliferation, and the NKX2.1/PIK3R3 axis might become a target in TGF-beta receptor-repressed lung adenocarcinoma PMID: 25371235
    15. p55gamma sequentially up-regulated p53 and p21, resulting in cell-cycle arrest in S phase; small-interfering RNA knockdown of either p53 or p21 blocked p55gamma-induced vascular smooth muscle cell growth arrest. PMID: 25388664
    16. Overexpression of PIK3R3 depends on SNAI2, inducing significant epithelial-to-mesenchymal transition (EMT). PMID: 24837077
    17. discovered that ERBB4 and S6K2 were the direct targets of miR-193a-3p and that PIK3R3 and mTOR were the direct targets of miR-193a-5p in non-small-cell lung cancer PMID: 24469061
    18. study shows that the p53/miR-148b/p55PIK axis has an important role in cell proliferation and tumor growth, and may represent a novel therapeutic target for treating cancers containing p53 mutations or losses. PMID: 24632606
    19. PIK3R3, ITGB1, ITGAL, and ITGA6, were involved in the regulation of actin cytoskeleton that link to triple-negative breast cancer migration. PMID: 24982892
    20. Containing the N24 of PI3K-p55PIK. PMID: 23904382
    21. our results show that the-PCNA interaction is important in regulating DNA synthesis and contributes to tumorigenesis. PMID: 23939377
    22. Data show that miR-7 inhibits the effects of TLR9 signaling on lung cancer cells through regulation of the PIK3R3/Akt pathway. PMID: 23135998
    23. PIK3R3 was up-regulated in gastric cancer and promoted cell cycle progression and proliferation. PMID: 22876838
    24. A fusion peptide containing the p55PIK amino terminus, TAT-N24, which not only inhibited cell proliferation, but also stimulated differentiation. PMID: 22722333
    25. IGF2-PIK3R3 signaling axis is involved in promoting the growth of a subclass of highly aggressive human glioblastomas that lack EGF receptor amplification. PMID: 17360667
    26. We have identified novel nonclassical mediators of the SREBP-1 response, including p55gamma, supporting the hypothesis that SREBP-1 regulates stress response and signaling genes. PMID: 17452746

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  • 蛋白家族：
    PI3K p85 subunit family
  • 組織特異性：
    Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle.
  • 數據庫鏈接：

    HGNC: 8981

    OMIM: 606076

    KEGG: hsa:8503

    STRING: 9606.ENSP00000262741

    UniGene: Hs.655387