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  4. MCM3 Recombinant Monoclonal Antibody

MCM3 Recombinant Monoclonal Antibody

  • 中文名稱：
    MCM3 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA261449A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Western Blot
      Positive WB detected in: NIH/3T3 whole cell lysate(30μg), HEK293 whole cell lysate(30μg)
      All lanes: MCM3 antibody at 1:1000
      Secondary
      Goat polyclonal to rabbit IgG at 1/40000 dilution
      Predicted band size: 91 kDa
      Observed band size: 120 kDa
      Exposure time：5min
    • IHC image of CSB-RA261449A0HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA261449A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Overlay Peak curve showing jurkat cells stained with CSB-RA261449A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    DNA replication licensing factor MCM3 (EC 3.6.4.12) (DNA polymerase alpha holoenzyme-associated protein P1) (P1-MCM3) (RLF subunit beta) (p102), MCM3
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Antibody
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    10A2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, IHC, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
  • 基因功能參考文獻：
    1. this study shows that MCM3 is a novel proliferation marker in oral squamous cell carcinoma PMID: 27258565
    2. High MCM3 expression is associated with bone metastasis and advanced human prostate cancer. PMID: 28424404
    3. reveal the Minichromosome Maintenance Complex to be a critical and directly regulated node within the miR-183 signaling network in MYCN-amplified neuroblastoma cells. Randomly selected MCMs 3 and 5 were experimentally confirmed as direct targets of miR-183. PMID: 27239679
    4. The results of our preliminary study emphasize the need for future research on MCM-3 as a sensitive proliferation marker, providing an alternative to Ki-67, in cases of various major salivary gland epithelial tumors in children and adolescents. PMID: 28547962
    5. These data establish new functions for KEAP1 within the nucleus and identify MCM3 as a novel substrate of the KEAP1-CUL3-RBX1 E3 ligase. PMID: 27621311
    6. The aim of this study is to analyze the immunoexpression of Ki67, p53, MCM3 and PCNA markers in epithelial remnants of dental follicles of impacted teeth and to identify a possible correlation between the immunoexpression of these markers in dentigerous cysts and keratocystic odontogenic tumors. PMID: 27516012
    7. High MCM-3 expression correlated with Cutaneous T-cell Lymphomas. PMID: 26504025
    8. normal DNA replication and replication checkpoint activation is regulated through the novel phosphorylation of MCM3 by Chk1 PMID: 25809478
    9. The relatively lower MCM3 protein expression in FVPTC. PMID: 23821456
    10. Expression of KB cell MCM3 was not affected by doxorubicin. PMID: 24344040
    11. present study aimed to investigate the relationship between expression of minichromosome maintenance proteins (MCM-3, MCM-7), metallothioneins (MT-I/II, MT-III), and Ki-67 in 103 ovarian cancer cases, mostly of the serous histological type PMID: 24324072
    12. High MCM3 expression is associated with mucoepidermoid carcinomas and adenoid cystic carcinomas in salivary gland tumors. PMID: 23886132
    13. Relatively lower MCM3 protein expression in follicular variant of papillary thyroid carcinoma comparing to classical type could be due to a different tumorigenic pathway favored in this type of tissue. PMID: 23821456
    14. These findings provide validation of the utility of MCM3 expression as an independent biomarker for prognostication of patients with primary melanoma PMID: 22805320
    15. excess of MCM3 up-regulates the phosphorylation of CHK1 Ser-345 and CDK2 Thr-14. PMID: 21965652
    16. MCM3 gene expression is decreased in follicular variant of papillary thyroid carcinoma, contrary to expectation. PMID: 21509594
    17. Using U373MG cells we found that HCMV IE86 protein was bound to Mcm3, but did not inhibit the cellular DNA synthesis. PMID: 20545442
    18. MCM3 may be a more reliable proliferation marker than Ki-67 in accessing the growth of tumor and evaluating tumor aggressiveness of papillary thyroid carcinoma. PMID: 19818763
    19. The MCM3 acetylase MCM3AP inhibits initiation, but not elongation, of DNA replication via interaction with this protein PMID: 12226073
    20. Elevated MCM3 protein expression is associated with neoplasms PMID: 15623617
    21. MCM3 is overexpressed in human astrocytic tumors and elicits a cancer-restricted humoral immune response in some patients with brain neoplasms PMID: 15671553
    22. ORC1 and Mcm3 proteins are required for DNA replication in protein extracts from human cells PMID: 16537544
    23. analysis of carboxyl-terminal MCM3 phosphorylation sites and their motifs PMID: 17244605
    24. These data reveal that CDK-dependent MCM3 phosphorylation contributes to the regulated formation of the MCM2-7 complex. PMID: 18524952

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  • 亞細胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    MCM family
  • 數據庫鏈接：

    HGNC: 6945

    OMIM: 602693

    KEGG: hsa:4172

    STRING: 9606.ENSP00000229854

    UniGene: Hs.179565