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  4. ATG13 Recombinant Monoclonal Antibody

ATG13 Recombinant Monoclonal Antibody

  • 中文名稱：
    ATG13 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA033362A0HU
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of ATG13 expression in HT-1080 cells using ATG13 antibody. Green, isotype control; red, ATG13.
    • Immunocytochemical staining of HT-1080 cells with ATG13 antibody. Nuclei were stained blue with DAPI; ATG13 was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
    • Western blotting analysis using ATG13 antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG13 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
  • 別名：
    ATG13; Autophagy Related 13; KIAA0652; Autophagy-Related Protein 13; ATG13 Autophagy Related 13 Homolog (S. Cerevisiae); ATG13 Autophagy Related 13 Homolog; PARATARG8
  • 反應種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human ATG13 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    21C2
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Autophagy factor required for autophagosome formation and mitophagy. Target of the TOR kinase signaling pathway that regulates autophagy through the control of the phosphorylation status of ATG13 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex. Through its regulation of ULK1 activity, plays a role in the regulation of the kinase activity of mTORC1 and cell proliferation.
  • 基因功能參考文獻：
    1. Specific affinity of the LC3 isoforms to the Atg13 LC3-interacting region is required for proper autophagosome formation. PMID: 24290141
    2. These data suggest that Atg101 is a novel Atg protein that functions together with ULK, Atg13 and FIP200. PMID: 19597335
    3. The gene product is the functional homologue of yeast Atg13, and required for autophagy in mammalian cells. PMID: 18936157
  • 亞細胞定位：
    Cytoplasm, cytosol. Preautophagosomal structure.
  • 蛋白家族：
    ATG13 family, Metazoan subfamily
  • 數據庫鏈接：

    HGNC: 29091

    OMIM: 615088

    KEGG: hsa:9776

    UniGene: Hs.127403