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  4. PIK3C2A Monoclonal Antibody

PIK3C2A Monoclonal Antibody

  • 中文名稱：
    PIK3C2A Monoclonal Antibody
  • 貨號：
    CSB-MA131474
  • 規格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of PIK3C2A expression in HepG2 cells using PIK3C2A antibody. Green, isotype control; red, PIK3C2A.
    • Immunocytochemical staining of HepG2 cells with PIK3C2A antibody. Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
    • Western blotting analysis using PIK3C2A antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIK3C2A antibody and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • 其他：

產品詳情

  • 產品名稱：
    Mouse anti-Homo sapiens (Human) PIK3C2A Monoclonal antibody
  • Uniprot No.：
  • 基因名：
  • 別名：
    PIK3C2A; Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Alpha; Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha; PI3K-C2alpha; Phosphoinositide-3-Kinase, Class 2, Alpha Polypeptide; Phosphoinositide 3-Kinase-C2-Alpha; PtdIns-3-Kinase C2 Subunit Alpha; PI3K-C2-Alpha; EC 2.7.1.154; Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha; Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 Alpha; C2-Containing Phosphatidylinositol Kinase; PI3-K-C2(ALPHA); EC 2.7.1.137; EC 2.7.1.153; PI3-K-C2A; EC 2.7.1; OCSKD; CPK
  • 宿主：
    Mouse
  • 反應種屬：
    Human
  • 免疫原：
    Recombinant Human PIK3C2A protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Mouse IgG2a
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    22A12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, WB, FC, ICC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:500-1:2500
    FC 1:200-1:2000
    ICC 1:100-1:1000
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function. Involved in the regulation of ciliogenesis and trafficking of ciliary components.
  • 基因功能參考文獻：
    1. PIK3C2A Polymorphism is associated with recurrence in non-small cell lung cancer. PMID: 28150169
    2. Four gene signature (PTEN, PIK3C2A, ITPA and BCL3) is an independent prognostic factors of both overall survival and disease-free survival in clear-cell renal-cell carcinoma. PMID: 27779101
    3. These results elucidated that PIK3C2A mRNA acts as a miR-124 decoy to regulate CD151 and to affect hepatocellular carcinoma malignant phenotypes. PMID: 27270320
    4. PIK3C2A was associated with bipolar disorder. PMID: 28195573
    5. Results present evidence that PI3K-C2alpha is critical in the proper maturation of endosomes, and for coordinating the use of endosomes as an additional membrane source during autophagy. PMID: 28910396
    6. miR-518a-5p was downregulated in imatinib-resistant gastrointestinal stromal tumors (GISTs), and the expression of miR-518a-5p was confirmed with good concordance between real-time PCR and miRNA microarray results. Luciferase reporter assays indicated that miR-518a-5p bound to the PIK3C2A 3'UTR. PMID: 26950487
    7. Therefore, PI3K-C2A is a factor important for human cytomegalovirus replication and has a role in the production of human cytomegalovirus virions. PMID: 27412598
    8. PI3KC2a knockdown results in rerouting of insulin signaling and pancreatic beta cell proliferation. PMID: 26387957
    9. MiR-206 regulates endothelial progenitor cell activities by targeting PIK3C2alpha expression.The role of PIK3C2alpha in endothelial progenitor cell function in coronary artery disease PMID: 26175229
    10. PI3KC2alpha has a role in regulating shear-dependent platelet adhesion via regulation of membrane structure, rather than acute signalling PMID: 25779105
    11. a model of S. flexneri dissemination is proposed in which the formation of vacuole-like protrusions is mediated by the PIK3C2A-dependent production of the signaling lipid phosphatidylinositol 3-phosphate in the protrusion membrane PMID: 25667265
    12. Data (including data from knockout mice) suggest that PI3KC2a participates in tumor angiogenesis. [REVIEW] PMID: 25233418
    13. These observations indicate that PI3K-C2alpha plays the pivotal role in TGFbeta receptor endocytosis and thereby Smad2/3 signaling, participating in angiogenic actions of TGFbeta. PMID: 25614622
    14. Class II enzyme PI3K-C2beta and the class IB isozyme p110gamma mainly regulate the S1P- and high density lipoprotein (HDL)-dependent endothelial cell (EC) migration and PI3K-C2alpha primarily controls EC survival PMID: 23320105
    15. PI3K-C2alpha plays the crucial role in S1P(1) internalization into the intracellular vesicular compartment, Rac1 activation on endosomes, and thereby migration through regulating vesicular trafficking in ECs PMID: 23192342
    16. These data suggest a general role for PI3KC2alpha in regulating physiologically relevant dynamin-independent internalization pathways. PMID: 21081650
    17. Phosphoinositide 3-kinase C2alpha was found to be differentially regulated by alpha(v)beta(3) and alpha(v)beta(5) integrin, and may represent a new regulatory pathway of cell migration downstream of integrin engagement. PMID: 12237112
    18. Nickel compounds induce HIF-1 transactivation and Cap43 protein expression through a PI-3K/Akt-dependent and p70(S6k)- independent pathway. PMID: 14729612
    19. VEGFR1 and VEGFR2 initiate a clonogenic response in myeloid leukemia cells that is PI3-kinase dependent. PMID: 15183893
    20. demonstrated that the isolated clathrin binding domain of Phosphoinositide 3-kinase C2alpha can drive clathrin lattice assembly and can independently modulate clathrin distribution and function when expressed in cells PMID: 16215232
    21. analysis of the PI specificities of PX domains and the mechanism by which the PI3K-C2alpha PX domain interacts with PtdIns(4,5)P(2)-containing membranes PMID: 17038310
    22. molecular linkage between PI3K-C2alpha and the microtubule motor machinery, with implications for membrane trafficking in intact cells PMID: 17110375
    23. phosphoinositide 3-kinase C2alpha has a role in insulin signaling PMID: 17644513
    24. Lowering the levels of PI3K-C2alpha in a number of cancer cell lines reduces their proliferation and cell viability. PMID: 18403640
    25. Taken together, these data suggest for the first time that in addition to class I PI3Ks in cancer, class II PIK3C2alpha can modulate hepatocellular carcinoma cell growth. PMID: 19591801

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  • 亞細胞定位：
    Cell membrane. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus. Cytoplasm. Golgi apparatus, trans-Golgi network.
  • 蛋白家族：
    PI3/PI4-kinase family
  • 組織特異性：
    Expressed in columnar and transitional epithelia, mononuclear cells, smooth muscle cells, and endothelial cells lining capillaries and small venules (at protein level). Ubiquitously expressed, with highest levels in heart, placenta and ovary, and lowest l
  • 數據庫鏈接：

    HGNC: 8971

    OMIM: 603601

    KEGG: hsa:5286

    STRING: 9606.ENSP00000265970

    UniGene: Hs.175343