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  4. EZR Recombinant Monoclonal Antibody

EZR Recombinant Monoclonal Antibody

  • 中文名稱：
    EZR重組抗體
  • 貨號：
    CSB-RA194531A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA194531A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    CSB-RA194531A0HU為EZR重組單克隆抗體，靶向人源ezrin蛋白（EZR）。EZR是ERM蛋白家族成員，通過連接細(xì)胞膜與細(xì)胞骨架調(diào)控細(xì)胞形態(tài)、黏附和遷移，在腫瘤發(fā)生、轉(zhuǎn)移及上皮-間質(zhì)轉(zhuǎn)化（EMT）過程中發(fā)揮重要作用。該抗體經(jīng)ELISA和免疫組化（IHC）嚴(yán)格驗(yàn)證，適用于石蠟包埋組織切片檢測，推薦稀釋比例為1:50-1:200。實(shí)驗(yàn)數(shù)據(jù)顯示，其能特異性識別EZR抗原表位，在IHC應(yīng)用中呈現(xiàn)清晰的膜/細(xì)胞質(zhì)定位信號且背景干擾低，批次間一致性優(yōu)異。適用于癌癥生物學(xué)研究（如乳腺癌、結(jié)腸癌的侵襲轉(zhuǎn)移機(jī)制）、細(xì)胞極性調(diào)控分析及發(fā)育生物學(xué)相關(guān)實(shí)驗(yàn)，支持通過IHC觀察腫瘤微環(huán)境中EZR蛋白分布特征，或結(jié)合ELISA技術(shù)定量檢測體外培養(yǎng)樣本中EZR表達(dá)水平。本產(chǎn)品采用重組表達(dá)技術(shù)制備，具備高靈敏度和穩(wěn)定性，為探究EZR在細(xì)胞信號傳導(dǎo)和疾病發(fā)生中的功能提供可靠工具。
  • Uniprot No.：
  • 基因名：
  • 別名：
    Ezrin (Cytovillin) (Villin-2) (p81), EZR, VIL2
  • 反應(yīng)種屬：
    Human
  • 免疫原：
    A synthesized peptide derived from human EZR
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    7F11
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
  • 基因功能參考文獻(xiàn)：
    1. detection of Ezrin and E-cadherin expression in cervical smears, could be a potential prognostic marker for identifying cervical lesions with high-risk of progression to invasive cervical cancer, and may help on the selection of an appropriate therapy or avoid unnecessary treatment PMID: 29587669
    2. Ezrin and myosin II play critical roles in enhancing line tension by promoting the formation of an actomyosin ring. PMID: 28643776
    3. these findings suggest that baicalein inhibits the proliferation, migration and invasion and induces apoptosis in Osteosarcoma (OS) cells by activating the miR183/Ezrin pathway, revealing a novel mechanism underlying antiOS effects of baicalein. PMID: 29845278
    4. Ezrin-anchored PKA phosphorylates serine 369 and 373 on connexin 43 to enhance gap junction assembly, communication, and cell fusion. PMID: 29259079
    5. High Ezrin expression is associated with osteosarcoma. PMID: 29656060
    6. L1CAM promotes esophageal squamous cell carcinoma tumorigenicity by upregulating ezrin expression. PMID: 28939985
    7. This is the first study to verify the relationship of the expression of RhoA and Ezrin proteins in vaginal tissue of Postmenopausal atrophic vagina. PMID: 28843271
    8. ezrin facilitates AQP2 endocytosis, thus linking the dynamic actin cytoskeleton network with AQP2 trafficking. PMID: 28754689
    9. FUT4/LeY was critical to the TAMs-mediated EMT; this process might be associated with the up-regulation of Ezrin phosphorylation by FUT4/LeY-mediated fucosylation PMID: 28423676
    10. CPI-17 drives Ras activity and tumorigenesis in melanomas in a two-fold way; inactivation of the tumor suppressor merlin and activation of the growth promoting ERM family. PMID: 27793041
    11. Data suggest that EGF induces colorectal cancer cells to undergo epithelial-mesenchymal transition, enhances their ability to invade/migrate, and promotes phosphorylation of Ezrin at Tyr353. (EGF = epidermal growth factor) PMID: 28535417
    12. Binding of phosphatidylinositol 4,5-biphosphate to ezrin induces a conformational change permitting the insertion of the LOK C-terminal domain to wedge apart the membrane and F-actin-binding domains of ezrin. The N-terminal LOK kinase domain can then access a site 40 residues distal from the consensus sequence that collectively direct phosphorylation of the appropriate threonine residue. PMID: 28430576
    13. The expression pattern and subcellular localization of ezrin and moesin correlate with clinicopathological variables such as patients' age, tumor grade and hormonal status. PMID: 28624994
    14. Ezrin represents a promising target for the development of strategies aimed at preventing the progression of cervical cancer. PMID: 26933912
    15. Ezrin S66 phosphorylation enhances filopodia formation, contributing to the regulation of invasion and metastasis of esophageal squamous cell carcinoma cells PMID: 28504189
    16. The results reveal a supportive role of ERMs in cortical activities during cytokinesis, and also provide insight into the selective mechanism that preferentially associates cytokinesis-relevant proteins with the division site. PMID: 28889652
    17. Ezrin protein expression is a promising biomarker in estimating the outcome of stage II colorectal cancer patients. When combined with microsatellite status its ability in predicting disease outcome is further improved PMID: 28953975
    18. Ezrin is down-regulated during cholangiocarcinogenesis, and its loss results in a more aggressive phenotype. PMID: 26791814
    19. A signature of ezrin-interacting proteins accurately predicts esophageal squamous cell carcinoma patient survival or tumor recurrence. PMID: 28603065
    20. The results of this meta-analysis suggest that ezrin positive immunoexpression confers a higher risk of recurrence and a worse survival in osteosarcoma patients. PMID: 23805177
    21. PM blebbing triggered SRF-mediated up-regulation of the metastasis-associated ERM protein Ezrin. Notably, Ezrin is sufficient and important to sustain bleb dynamics for cell-in-cell invasion when SRF is suppressed. PMID: 28774893
    22. EZR is a novel biomarker in terms of invasion among the 3 subtypes of NFPAs, and it is a promising guide for therapeutic decision making as well. PMID: 28093347
    23. Increased ezrin and HER2 expression in patients with salivary gland carcinomas represents a high-grade histopathological subtype. PMID: 28300573
    24. SMYD3 enhances tumorigenicity in esophageal squamous cell carcinoma by enhancing transcription of ezrin and LOXL2, which are involved in proliferation, migration, and invasion. PMID: 26980013
    25. 3-dimensional cell cultures were found to mimic different tumor sites and be applicable as a model. The in vitro results concur with the clinical specimen analysis, suggesting that in ovarian carcinoma, the role of ezrin in disease progression is more pronounced than that of p130Cas. PMID: 27622508
    26. The expression of ezrin was up-regulated and significantly associated with the stage, lymph node involvement and distant metastasis PMID: 28261953
    27. there were significant decreases in intercellular adhesion molecules 1 (ICAM1), ezrin (EZR), mitogen-activated protein kinase kinase 2 (MAP2K2), and nitric oxide synthase 3 (NOS3) gene expressions in metabolic syndrome patients. PMID: 26956845
    28. Immunohistochemistry staining for ezrin, was similar in AFX and UPS tumors. PMID: 28079637
    29. Ezrin and HER2/neu are overexpressed and coexpressed in osteosarcoma with adverse prognostic features such as high grade. Therefore, ezrin and HER2/neu could be potential prognostic markers and treatment targets for osteosarcoma PMID: 26067138
    30. Study indicates the usual relationship between estrogen and ezrin induction is abridged. Study suggests that changes in ezrin may be associated with the development of the invasive phenotype and penetration of the basement membrane. PMID: 27688241
    31. The present study showed over-expression of ezrin and moesin in colorectal carcinoma PMID: 27042764
    32. study indicates that the presence of autoantibodies against Ezrin is significantly associated with ESCC PMID: 28298808
    33. Ezrin protein is highly expressed in human PHC tissue which can be used for the prediction of metastasis disease. PMID: 28230040
    34. results show that the activation of the ezrin-pAkt signaling axis is associated with the more aggressive clinicopathological features of PPA compared with LPA PMID: 27059464
    35. Ezrin and p65 interactions in MDA-MB-231 cells were confirmed using co-immunoprecipitation. PMID: 27420986
    36. the distribution of NHERF1 in ovarian cancer and reveals a different regulation of NHERF1 and EZRIN expression in ovarian tumors which represents the complexity of the molecular changes of this disease PMID: 27823775
    37. Phosphorylation of ezrin together with its binding to phosphatidylinositol-4,5-bisphosphate tethers the F508del CFTR to the actin cytoskeleton, stabilizing it on the apical membrane and rescuing the sub-membrane compartmentalization of cAMP and activated PKA. PMID: 26823603
    38. Data show that gene silence of ezrin inhibits the proliferation and invasion of prostate cancer PC-3 cells, meanwhile the level of E-cadherin is upregulated and N-cadherin is downregulated. PMID: 27371852
    39. Knockdown of ezrin in HUVECs significantly induced the morphogenetic changes and cytoskeletal reorganization of the transfected cells, and also reduced cell migration and angiogenesis capacity in vitro. PMID: 27072970
    40. High EZRIN expression is associated with prostate cancer. PMID: 26799186
    41. Elevated Ezrin expression is associated with a poor prognosis in a variety of solid tumors. PMID: 26632332
    42. These findings suggest that ezrin-EGFR interaction augments oncogenic functions of EGFR and that targeting ezrin may provide a potential novel approach to overcome erlotinib resistance in non-small cell lung cancer cells PMID: 26936397
    43. The value of ezrin expression as a prognostic biomarker is further consolidated in urothelial cancer. PMID: 25278252
    44. Suggest a role for ezrin in advanced glycation end product-induced podocyte damage. PMID: 26032400
    45. Phospho-Ezrin/Radixin/Moesin (ERM) inhibit cell adhesion, and therefore, dephosphorylation of ERM proteins is essential for cell adhesion.Phospho-ERM induce formation and/or maintenance of spherical cell shape. PMID: 26555866
    46. Activation of liver PKCs during cholestasis leads to Ezrin Thr567 phosphorylation resulting in MRP2 internalization and degradation where ubiquitin ligase E3 GP78 is involved. PMID: 26212029
    47. Data indicate that a quinoline-based small molecule, NSC305787, directly binds to ezrin and inhibits its functions. PMID: 26358752
    48. We identified and confirmed that Fra-1 affected the expression level of CTTN and EZR in vitro through LC-MS/MS analyses and western blot technology. PMID: 26330014
    49. Data show that both Ezrin and SIX1 proteins are highly expressed in alpha fetoprotein-negative hepatocellular carcinoma (HCC) and significantly related with the TNM stage. PMID: 26927385
    50. it was found that expression of miR-96 was negatively correlated with the metastatic ability of renal cell carcinoma, and that downregulation of miR-96 could suppress the invasion of renal cancer cell via downregulation of Ezrin expression. PMID: 26419932

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  • 亞細(xì)胞定位：
    Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cell projection, microvillus.
  • 組織特異性：
    Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component
  • 數(shù)據(jù)庫鏈接：

    HGNC: 12691

    OMIM: 123900

    KEGG: hsa:7430

    STRING: 9606.ENSP00000338934

    UniGene: Hs.487027