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  4. DLAT Recombinant Monoclonal Antibody

DLAT Recombinant Monoclonal Antibody

  • 中文名稱：
    DLAT Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA238917A0HU
  • 規(guī)格：
    ￥1320
  • 圖片：
    • Flow cytometric analysis of DLAT expression in HepG2 cells using DLAT antibody. Green, isotype control; red, DLAT.
    • Immunocytochemical staining of HepG2 cells with DLAT Antibody antibody. Nuclei were stained blue with DAPI; DLAT Antibody was stained magenta with Alexa Fluor? 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
    • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using DLAT antibody. Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
    • Western blotting analysis using DLAT antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DLAT antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • 其他：

產(chǎn)品詳情

  • Uniprot No.：
  • 基因名：
    DLAT
  • 別名：
    DLAT; Dihydrolipoamide S-Acetyltransferase; PDC-E2; DLTA; E2; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate; Dehydrogenase Complex, Mitochondrial; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; E2 Component Of Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex Component E2; M2 Antigen Complex 70 KDa Subunit; EC 2.3.1.12; PDCE2; PBC; Dihydrolipoyllysine-Residue Acetyltransferase; EC 2.3.1
  • 反應(yīng)種屬：
    Human, Mouse, Rat
  • 免疫原：
    Recombinant Human DLAT protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標(biāo)記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    7F12
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
  • 產(chǎn)品提供形式：
    Liquid
  • 應(yīng)用范圍：
    ELISA, WB, FC, ICC, IHC
  • 推薦稀釋比：
    Application Recommended Dilution
    WB 1:1000-1:5000
    FC 1:200-1:2000
    ICC 1:100-1:1000
    IHC 1:100-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價(jià)

靶點(diǎn)詳情

  • 功能：
    The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
  • 基因功能參考文獻(xiàn)：
    1. DLAT interacts with C1QBP in mitochondria. PMID: 26753982
    2. ongoing activation of PDCE2-specific B-cells in primary biliary cirrhosis PMID: 25043065
    3. Results show that DLAT and ACAT2 as upstream acetyltransferases of K76 and K294 in 6PGD protein. PMID: 25042803
    4. These findings were used to identify potentially antigenic sequences within PDC-E2 (an important hepatic autoantigen) that contain a DR0801 motif. PMID: 23543758
    5. Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly PMID: 20361979
    6. Data suggest that the catalytic site of pyruvate dehydrogenase complex E2 rather than the previously reported lipoyl binding peptide may contain immunodominant epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. PMID: 20180236
    7. a novel HLA-A*0201-restricted epitope PDC-E2 165 to 174 was defined in patients with primary biliary cirrhosis. PMID: 12395322
    8. study of facilitated interaction between the pyruvate dehydrogenase kinase isoform 2 and the dihydrolipoyl acetyltransferase PMID: 12816949
    9. model of the pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and binding of the E1 and E3 components PMID: 14638692
    10. Having found that there is an excellent and almost unique match between the PDC-E2 autoepitope and a sequence in mycobacterial hsp65s, we tested the corresponding peptides for cross-reactivity using sera from 90 Spanish and British PBC patients. PMID: 15120760
    11. This study report two unrelated patients with pyruvate dehydrogenase deficiency caused by defects in the E2 subunit. PMID: 16049940
    12. epitope specificity of these PDC-E2 autoantibodies was distinctive suggesting that the mechanisms leading to loss of tolerance in the transplantation patients are distinct from primary biliary cirrhosis PMID: 17068145
    13. Species specificity in the interaction between hE1beta and hE2 in pyruvate dehydrogenase complex. PMID: 18206651
    14. tissue specificity of the autoimmune injury in primary biliary cirrhosis is a consequence of the unique characteristics of HIBECs during apoptosis and can be explained by exposure to immune system of intact immunoreactive PDC-E2 within apoptotic blebs. PMID: 19185000

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  • 相關(guān)疾病：
    Pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency)
  • 亞細(xì)胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    2-oxoacid dehydrogenase family
  • 數(shù)據(jù)庫鏈接：

    HGNC: 2896

    OMIM: 245348

    KEGG: hsa:1737

    STRING: 9606.ENSP00000280346

    UniGene: Hs.335551