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  4. ABCF1 Recombinant Monoclonal Antibody

ABCF1 Recombinant Monoclonal Antibody

  • 中文名稱：
    ABCF1 Recombinant Monoclonal Antibody
  • 貨號：
    CSB-RA216952A0HU
  • 規格：
    ￥1320
  • 圖片：
    • IHC image of CSB-RA216952A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-RA216952A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of HeLa cell with CSB-RA216952A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
    • Overlay Peak curve showing Hela cells stained with CSB-RA216952A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 其他：

產品詳情

  • Uniprot No.：
  • 基因名：
    ABCF1
  • 別名：
    ABC27 antibody; ABC50 antibody; ABCF1 antibody; ABCF1_HUMAN antibody; ATP binding cassette 50 (TNF alpha stimulated) antibody; ATP binding cassette 50 antibody; ATP binding cassette sub family F member 1 antibody; ATP binding cassette; sub family F (GCN20); member 1 antibody; ATP-binding cassette 50 antibody; ATP-binding cassette sub-family F member 1 antibody; AU041969 antibody; D17Wsu166e antibody; EST123147 antibody; GCN20 antibody; TNF alpha stimulated ABC protein antibody; TNF-alpha-stimulated ABC protein antibody; TNFalpha inducible ATP binding protein antibody; wu:fb79c06 antibody; wu:fc39a05 antibody; wu:fj94a08 antibody; zgc:85667 antibody
  • 反應種屬：
    Human
  • 免疫原：
    A synthesized peptide from human ABCF1 protein
  • 免疫原種屬：
    Homo sapiens (Human)
  • 標記方式：
    Non-conjugated
  • 克隆類型：
    Monoclonal
  • 抗體亞型：
    Rabbit IgG
  • 純化方式：
    Affinity-chromatography
  • 克隆號：
    8G6
  • 濃度：
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液：
    Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式：
    Liquid
  • 應用范圍：
    ELISA, IHC, IF, FC
  • 推薦稀釋比：
    Application Recommended Dilution
    IHC 1:50-1:200
    IF 1:50-1:200
    FC 1:50-1:200
  • Protocols：
  • 儲存條件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 用途：
    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

  • 功能：
    Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
  • 基因功能參考文獻：
    1. The high copy number variant in the ABCF1 gene was the strongest risk factor for gout. PMID: 28405828
    2. ABCF1 extrinsically regulates retinal pigment epithelial cell phagocytosis of shed photoreceptor outer segments. PMID: 25904329
    3. Elevated microRNA-23a Expression Enhances the Chemoresistance of Colorectal Cancer Cells with Microsatellite Instability to 5-Fluorouracil by Directly Targeting ABCF1 PMID: 25929864
    4. expression of ABC50 mutants, which cannot hydrolyse ATP, decreases general translation and relaxes the discrimination against the use of non-AUG codons at translation start sites. PMID: 25597744
    5. A rare copy number variation of the ABCF1 gene was detected among dengue patients from Malaysia. PMID: 24634119
    6. The results suggested that SNPs at PRR3 and ABCF1 genes and the haplotype composed by SNPs at GNL1 and PRR3 between the HLA-A and HLA-C genes tended to predict Graves ophthalmology in a gender-dependent manner in patients with Graves disease in Taiwan. PMID: 24908204
    7. ABCF1 showed a significant increase in expression after escitalopram treatment. PMID: 23719290
    8. ABCF1 is a critical protein that associates with viral double-stranded DNA. PMID: 23263557
    9. The two critical HLA regions for susceptibility to autoimmune pancreatitis are limited to the HLA-DRB1*0405-DQB1*0401 in the class II and the ABCF1in the class I regions. PMID: 17119950
    10. ABC50 N-terminal region interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2 PMID: 17894550
    11. ABC50 plays a key role in translation initiation and has functions that are distinct from those of other non-membrane ABC proteins. PMID: 19570978

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  • 亞細胞定位：
    [Isoform 2]: Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
  • 蛋白家族：
    ABC transporter superfamily, ABCF family, EF3 subfamily
  • 組織特異性：
    Ubiquitous.
  • 數據庫鏈接：

    HGNC: 70

    OMIM: 603429

    KEGG: hsa:23

    STRING: 9606.ENSP00000313603

    UniGene: Hs.655285