Rat platelet-derived growth factor-BB,PDGF-BB ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠血小板衍生生長因子BB(PDGF-BB)酶聯(lián)免疫試劑盒（CSB-E08924r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的PDGF-BB含量。PDGF-BB屬于血小板衍生生長因子，在細胞的生長、增殖和分化中發(fā)揮重要作用。其異常表達與腫瘤血管生成、纖維化疾病等多種病理過程相關。研究通過抑制其信號通路，阻斷細胞增殖與遷移，有望為相關疾病治療提供新策略。試劑盒檢測范圍為39.063 pg/mL-2500 pg/mL，該產(chǎn)品適用于基礎科研領域，如細胞模型中生長因子調控機制研究、動物疾病模型中PDGF - BB動態(tài)變化的定量分析，以及組織微環(huán)境修復相關實驗的分子水平檢測，為探索心血管疾病、組織再生及病理損傷等研究方向提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

PDGF-BB
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

39.063 pg/mL-2500 pg/mL
靈敏度：

9.77 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	104
	Range %	101-107
1:2	Average %	97
	Range %	94-100
1:4	Average %	88
	Range %	83-90
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	101-109
EDTA plasma (n=4)	110	107-113

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.