Mouse platelet-derived growth factor-BB,PDGF-BB ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠血小板衍生生長因子BB(PDGF-BB)酶聯免疫試劑盒（CSB-E08925m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的PDGF-BB含量。PDGF-BB 是血小板衍生生長因子異構體。其背景在于它在細胞生長、增殖等生理過程有關鍵作用。研究機制主要圍繞其與受體結合，激活下游信號通路，在腫瘤血管生成、纖維化疾病發展等病理過程有重要影響，也是藥物研發熱門靶點。試劑盒檢測范圍為0.18 ng/mL-12 ng/mL，科研人員可將其應用于小鼠模型中的炎癥反應研究、血管生成機制探索或組織再生相關實驗，也可用于評估藥物干預或基因修飾對 PDGF-BB 表達的影響。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

PDGF-BB
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.18 ng/mL-12 ng/mL
靈敏度：

0.047 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-88
1:2	Average %	94
	Range %	89-98
1:4	Average %	104
	Range %	97-109
1:8	Average %	99
	Range %	93-105

回收率：

The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	89	82-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.