Rat Pentosidine ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

大鼠戊糖素(Pentosidine)酶聯(lián)免疫試劑盒（CSB-E09416r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的Pentosidine含量。Pentosidine是一種晚期糖基化終末產(chǎn)物（AGEs）。它與多種疾病相關(guān)，如糖尿病并發(fā)癥、心血管疾病等。其研究機(jī)制主要聚焦在它可介導(dǎo)氧化應(yīng)激和炎癥反應(yīng)，促進(jìn)細(xì)胞功能障礙和組織損傷，是研究疾病發(fā)病機(jī)制及尋找潛在治療靶點(diǎn)的重要方向。試劑盒檢測范圍為15.6 pmol/mL-1000 pmol/mL，在代謝性疾病機(jī)制、氧化應(yīng)激研究及抗衰老藥物開發(fā)等科研領(lǐng)域具有重要價值；尤其適用于糖尿病模型大鼠的血清/血漿樣本分析，或肝臟、腎臟等組織的勻漿液檢測；為研究戊糖素在慢性疾病發(fā)展中的作用機(jī)制、評估干預(yù)措施效果提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

Pentosidine
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pmol/mL-1000 pmol/mL
靈敏度：

3.9 pmol/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-89
1:2	Average %	89
	Range %	85-93
1:4	Average %	96
	Range %	92-100
1:8	Average %	92
	Range %	86-97

回收率：

The recovery of rat Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	96-105
EDTA plasma (n=4)	90	85-95

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.