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  4. Human Pentosidine ELISA Kit
  • 中文名稱(chēng)：
    人戊糖素(Pentosidine)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E09415h
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷(xiāo)：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    人戊糖素(Pentosidine)酶聯(lián)免疫試劑盒（CSB-E09415h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、尿液樣本中的Pentosidine含量。Pentosidine是一個(gè)靶點(diǎn)，它屬于晚期糖基化終末產(chǎn)物（AGEs）。在體內(nèi)，它主要通過(guò)美拉德反應(yīng)形成并逐漸積累。研究機(jī)制方面，它與糖尿病并發(fā)癥、衰老等密切相關(guān)，可引發(fā)氧化應(yīng)激和炎癥反應(yīng)，常作為評(píng)估相關(guān)疾病風(fēng)險(xiǎn)和進(jìn)程的生物標(biāo)志物。試劑盒檢測(cè)范圍為31.25 pmol/mL-2000 pmol/mL，該產(chǎn)品適用于基礎(chǔ)醫(yī)學(xué)研究、疾病機(jī)制探索、藥物干預(yù)效果評(píng)估等科研場(chǎng)景，為代謝性疾病、衰老相關(guān)疾病及骨關(guān)節(jié)健康研究提供可靠的生物標(biāo)志物檢測(cè)方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫(xiě)：
    Pentosidine
  • 種屬：
    Homo sapiens (Human)
  • 樣本類(lèi)型：
    serum, plasma, tissue homogenates, urine
  • 檢測(cè)范圍：
    31.25 pmol/mL-2000 pmol/mL
  • 靈敏度：
    7.81 pmol/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Others
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human Pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %94
    Range %81-102
    1:2Average %95
    Range %84-105
    1:4Average %104
    Range %91-111
    1:8Average %95
    Range %80-103
  • 回收率：
    The recovery of human Pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9285-105
    EDTA plasma (n=4)9488-105
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pmol/mlOD1OD2AverageCorrected
    20002.677 2.697 2.687 2.537
    10001.843 1.864 1.854 1.704
    5001.163 1.153 1.158 1.008
    2500.742 0.754 0.748 0.598
    1250.447 0.461 0.454 0.304
    62.50.347 0.359 0.353 0.203
    31.250.268 0.262 0.265 0.115
    00.154 0.146 0.150 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

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