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  4. Rat Interleukin 3,IL-3 ELISA KIT

Rat Interleukin 3,IL-3 ELISA KIT

  • 中文名稱：
    大鼠白介素3(IL-3)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-E07436r
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素3(IL-3)酶聯(lián)免疫試劑盒（CSB-E07436r）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL3含量。IL3，即白細(xì)胞介素-3，是一種由激活的T細(xì)胞和其他細(xì)胞產(chǎn)生的細(xì)胞因子，主要在造血過程中發(fā)揮作用，調(diào)控粒細(xì)胞和單核細(xì)胞-巨噬細(xì)胞的產(chǎn)生、分化和功能。其研究機(jī)制涉及IL3如何通過激活細(xì)胞內(nèi)信號(hào)通路，如Ras-Raf-ERK和JAK/STAT，來促進(jìn)造血細(xì)胞的生長(zhǎng)和分化。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，適用于科研領(lǐng)域中對(duì)IL-3表達(dá)水平的研究，例如在造血系統(tǒng)疾病模型、感染性疾病動(dòng)物實(shí)驗(yàn)或免疫相關(guān)病理機(jī)制探討中，通過檢測(cè)血液或組織樣本中的IL-3濃度變化，揭示其在炎癥反應(yīng)、細(xì)胞分化及免疫調(diào)控中的作用規(guī)律。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Il3 ELISA Kit; Il-3Interleukin-3 ELISA Kit; IL-3 ELISA Kit; Hematopoietic growth factor ELISA Kit; Mast cell growth factor ELISA Kit; MCGF ELISA Kit; Multipotential colony-stimulating factor ELISA Kit; P-cell-stimulating factor ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍：
    3.12 pg/mL-200 pg/mL
  • 靈敏度：
    0.78 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %94
    Range %90-98
    1:2Average %100
    Range %95-113
    1:4Average %91
    Range %85-97
    1:8Average %97
    Range %92-104
  • 回收率：
    The recovery of rat IL-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9085-94
    EDTA plasma (n=4)9387-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    2002.436 2.276 2.356 2.214
    1001.975 2.014 1.995 1.853
    501.435 1.372 1.404 1.262
    250.868 0.913 0.891 0.749
    12.50.553 0.519 0.536 0.394
    6.250.387 0.407 0.397 0.255
    3.120.285 0.276 0.281 0.139
    00.144 0.140 0.142
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.; This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-3 family
  • 組織特異性：
    Activated T-cells, mast cells, natural killer cells.
  • 數(shù)據(jù)庫鏈接：

    UniGene: Rn.10652