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  4. Mouse interferon alpha-2(IFNA2) ELISA kit

Mouse interferon alpha-2(IFNA2) ELISA kit

  • 中文名稱：
    小鼠干擾素α2(IFNA2)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011038MO
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    小鼠干擾素α2(IFNA2)酶聯(lián)免疫試劑盒（CSB-EL011038MO）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的IFNA2含量。IFNA2 即干擾素α2，是一種細(xì)胞因子。它具有抗病毒、抗腫瘤和免疫調(diào)節(jié)等作用。其研究機(jī)制主要在于它能與細(xì)胞表面受體結(jié)合，激活信號(hào)通路，誘導(dǎo)下游多種抗病毒和免疫相關(guān)基因表達(dá)，從而發(fā)揮生物學(xué)效應(yīng)。試劑盒檢測(cè)范圍為9.38 pg/mL-600 pg/mL，適用于體外實(shí)驗(yàn)中IFNA2表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)，例如病毒學(xué)研究中宿主免疫應(yīng)答評(píng)估、免疫調(diào)節(jié)機(jī)制探索或炎癥相關(guān)疾病模型構(gòu)建等科研場(chǎng)景。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
  • 別名：
    Ifna2Interferon alpha-2 ELISA Kit; IFN-alpha-2 ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates, cell lysates
  • 檢測(cè)范圍：
    9.38 pg/mL-600 pg/mL
  • 靈敏度：
    2.35 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測(cè)波長(zhǎng)：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測(cè)定原理：
    quantitative
  • 測(cè)定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse IFNA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 90
    Range % 85-94
    1:2 Average % 102
    Range % 98-107
    1:4 Average % 84
    Range % 80-88
    1:8 Average % 95
    Range % 95-99
  • 回收率：
    The recovery of mouse IFNA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 93 89-98
    EDTA plasma (n=4) 98 94-102
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    600 2.651 2.536 2.594 2.440
    300 2.049 1.928 1.989 1.835
    150 1.376 1.315 1.346 1.192
    75 0.892 0.952 0.922 0.768
    37.5 0.568 0.529 0.549 0.395
    18.75 0.374 0.364 0.369 0.215
    9.4 0.237 0.241 0.239 0.085
    0 0.150 0.157 0.154 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Produced by macrophages, IFN-alpha have antiviral activities.
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    Alpha/beta interferon family
  • 數(shù)據(jù)庫(kù)鏈接：