PIK3CA

PIK3CA，即磷脂酰肌醇4,5-二磷酸3-激酶催化亞基α，別名PI3K-alpha。PIK3CA由人類PIK3CA基因編碼，該基因位于3號染色體的q26.3區(qū)域，包含21個(gè)外顯子，編碼1068種氨基酸，產(chǎn)生一組長124kD的蛋白。PIK3CA的作用機(jī)制涉及催化膜脂磷脂酰肌醇-4,5-二磷酸[PI(4,5)P2]到第二信使PI(3,4,5)P3（也被稱為PIP3）的轉(zhuǎn)化，激活A(yù)KT-mTOR信號通路，促進(jìn)細(xì)胞存活、增殖、生長和運(yùn)動(dòng)。PIK3CA是癌癥中最常見的突變基因之一，其激活突變在乳腺癌、子宮內(nèi)膜癌、膀胱癌、結(jié)腸直腸癌等多種癌癥中均有發(fā)現(xiàn)，參與腫瘤發(fā)生、進(jìn)展及轉(zhuǎn)移定植。PIK3CA基因的突變導(dǎo)致PI3K/AKT通路水平上調(diào)，進(jìn)而參與腫瘤發(fā)生。

熱銷產(chǎn)品

Recombinant Human?Phosphatidylinositol?4,5-bisphosphate?3-kinase?catalytic?subunit?alpha?isoform (PIK3CA), partial (CSB-EP017998HU)

驗(yàn)證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

PIK3CA Recombinant Monoclonal Antibody (CSB-RA578819A0HU)

驗(yàn)證數(shù)據(jù)

Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate, MCF-7 whole cell lysate
All lanes: PIK3CA antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 125 kDa
Observed band size: 110 kDa

Immunofluorescence staining of Hela Cells with CSB-RA578819A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

PIK3CA Antibody

PIK3CA Protein

PIK3CA ELISA Kit

PIK3CA Antibodies

PIK3CA for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA017998GA01HU	PIK3CA Antibody	Human,Mouse,Rat	ELISA,WB
CSB-PA003761	PIK3CA Antibody	Human,Mouse	WB, IHC, ELISA
CSB-PA017998LA01HU	PIK3CA Antibody	Human	ELISA, IHC, IF
CSB-PA017998LC01HU	PIK3CA Antibody, FITC conjugated	Human
CSB-PA017998LD01HU	PIK3CA Antibody, Biotin conjugated	Human	ELISA
CSB-RA578819A0HU	PIK3CA Recombinant Monoclonal Antibody	Human, Mouse	ELISA, WB, IF

PIK3CA Proteins

PIK3CA Proteins for Bos taurus (Bovine)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP017998BO CSB-EP017998BO CSB-BP017998BO CSB-MP017998BO CSB-EP017998BO-B	Recombinant Bovine Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

PIK3CA Proteins for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP017998HU CSB-BP017998HU CSB-MP017998HU CSB-EP017998HU-B	Recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA), partial	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli
CSB-EP017998HU	Recombinant Human?Phosphatidylinositol?4,5-bisphosphate?3-kinase?catalytic?subunit?alpha?isoform (PIK3CA), partial	E.coli

PIK3CA Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP017998MO CSB-EP017998MO CSB-BP017998MO CSB-MP017998MO CSB-EP017998MO-B	Recombinant Mouse Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (Pik3ca), partial	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli