(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Human F11 at 2 μg/ml can bind Anti-F11 recombinant antibody (CSB-RA007916MA1HU). The EC₅₀ is 0.2442-0.2820 ng/mL.

Activity
Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >300 pmol/min/μg.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 at 2 μg/ml can bind Anti-F11 recombinant antibody (CSB-RA007916MA1HU). The EC₅₀ is 0.5654-0.6444 ng/mL.

Activity
Measured by its ability to cleave the fluorogenic peptide substrate, t-butyloxycarbonyl-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin (Boc-IEGR-AMC). The specific activity is >150 pmol/min/μg.

The purity of TrEMBL was greater than 95% as determined by SEC-HPLC

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

The Binding Activity of Macaca mulatta F11 with Anti-F11 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Macaca mulatta F11 (CSB-MP5433MOW) at 2 μg/mL can bind Anti-F11 recombinant antibody. The EC₅₀ is 0.5654-0.6444 ng/mL.

The Binding Activity of Human F11 with Anti-F11 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human F11 (CSB-MP010949HU) at 2 μg/mL can bind Anti-F11 recombinant antibody. The EC₅₀ is 0.2442-0.2820 ng/mL.