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  4. Recombinant Arabidopsis thaliana Probable aquaporin NIP5-1 (NIP5-1)

Recombinant Arabidopsis thaliana Probable aquaporin NIP5-1 (NIP5-1)

  • 中文名稱：
    擬南芥NIP5-1重組蛋白
  • 貨號：
    CSB-CF890454DOA
  • 規格：
  • 來源：
    in vitro E.coli expression system
  • 其他：

產品詳情

  • 基因名：
    NIP5-1
  • Uniprot No.：
  • 別名：
    NIP5-1; NLM6; At4g10380; F24G24.180; Probable aquaporin NIP5-1; NOD26-like intrinsic protein 5-1; AtNIP5;1; Nodulin-26-like major intrinsic protein 6; NodLikeMip6; Protein NLM6
  • 種屬：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白長度：
    full length protein
  • 表達區域：
    1-304
  • 氨基酸序列
    MAPPEAEVGAVMVMAPPTPGTPGTPGGPLITGMRVDSMSFDHRKPTPRCKCLPVMGSTWGQHDTCFTDFPSPDVSLTRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISGAHLNPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAFALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPAVASGNYRSLWVYLVAPTLGAISGAAVYTGVKLNDSVTDPPRPVRSFRR
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標簽：
    N-terminal 10xHis-tagged
  • 產品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 儲存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事項：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產品評價

靶點詳情

  • 功能：
    Boric acid transporter. Low water transport activity. Plays an important role as plasma membrane boric acid channel for the boron uptake required for plant growth and development under boron limitation.
  • 基因功能參考文獻：
    1. The results demonstrate that the polar localization of NIP5;1 is maintained by clathrin-mediated endocytosis, is dependent on phosphorylation in the TPG repeat, and is necessary for the efficient transport of boron in roots. PMID: 28341806
    2. The direct root uptake of urea was increased under boron deficiency, in both the high and low affinity range. This activity was entirely mediated by the NIP5;1 channel. PMID: 25957355
    3. Rough mapping and whole-genome sequencing identified the mutant with altered localization of NIP5;1 as an allele of UDP-glucose 4-epimerase 4. PMID: 24343997
    4. The 5' UTR controls boron-dependent NIP5;1 mRNA degradation and that NIP5;1 mRNA degradation is important for plant acclimation to high-boron conditions. PMID: 21908722
    5. The residue at the H5 helix position of the aromatic/arginine filter of both OsLsi1 and AtNIP5;1 plays a key role in the permeability to silicon and boron, but there is a relatively low selectivity for arsenite. PMID: 21586431
    6. NIP5;1 functions as a boric acid channel for Boron uptake and is crucial for plant growth under Boron limitation in Arabidopsis. PMID: 16679457
    7. Enhanced expression of NIP5;1 resulted in improved root elongation under boron-limiting conditions. PMID: 19017629
    8. Nodulin26-like Intrinsic Proteins (NIPs) AtNIP5;1 and AtNIP6;1 from A. thaliana, OsNIP2;1 and OsNIP3;2 from O. sativa, and LjNIP5;1 and LjNIP6;1 from Lotus japonicus are bi-directional As(III) channels. PMID: 18544156

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  • 亞細胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    MIP/aquaporin (TC 1.A.8) family, NIP (TC 1.A.8.12) subfamily
  • 組織特異性：
    Expressed in rosette leaves.
  • 數據庫鏈接：

    KEGG: ath:AT4G10380

    STRING: 3702.AT4G10380.1

    UniGene: At.33633