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  4. Recombinant Mouse Hexokinase-2 (Hk2), partial

Recombinant Mouse Hexokinase-2 (Hk2), partial

  • 中文名稱：
    Recombinant Mouse Hexokinase-2 (Hk2), partial
  • 品名簡(jiǎn)稱：
    Recombinant Mouse Hk2 protein, partial
  • 貨號(hào)：
    CSB-EP010469MO
  • 說(shuō)明書：
  • 規(guī)格：
    ￥1344
  • 圖片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

產(chǎn)品詳情

  • 純度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
  • Uniprot No.：
  • 別名：
    Hexokinase type II
  • 種屬：
    Mus musculus (Mouse)
  • 蛋白長(zhǎng)度：
    Partial
  • 來(lái)源：
    E.coli
  • 分子量：
    56.6 kDa
  • 表達(dá)區(qū)域：
    16-458aa
  • 氨基酸序列
    QNQVQKVDQYLYHMRLSDETLLEISRRFRKEMEKGLGATTHPTAAVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLRVRVTDNGLQRVEMENQIYAIPEDIMRGSGTQLFDHIAECLANFMDKLQIKEKKLPLGFTFSFPCHQTKLDESFLVSWTKGFKSSGVEGRDVVDLIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDQNCEIGLIVGTGSNACYMEEMRHIDMVEGDEGRMCINMEWGAFGDDGTLNDIRTEFDREIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKAELLFQGKLSPELLTTGSFETKDVSDIEDDKDGIQKAYQILVRLGLSPLQEDCVATHRICQIVSTRSASLCAATLAAVLWRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKAVRRLVPDCDVRFLRSEDGSGKGAAMVTA
    Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
    If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
  • 蛋白標(biāo)簽：
    C-terminal 6xHis-tagged
  • 產(chǎn)品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 緩沖液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 復(fù)溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 儲(chǔ)存條件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保質(zhì)期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 貨期：
    Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事項(xiàng)：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate. Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis.
  • 基因功能參考文獻(xiàn)：
    1. Kaempferol inhibited mitochondrial fission and maintained mitochondrial HK-II through activation of Akt, and thereby protected neurons from succinate-mediated ischemi injury. PMID: 28634116
    2. In high-fat diet fed mice, oral administration of mangiferin induced Akt phosphorylation, increased HK-II binding to mitochondria and resultantly protected vessel endothelial function, demonstrating its protective effect on endothelial integrity in vivo. PMID: 28478227
    3. Studied melatonin's role in microvascular ischemia/reperfusion injury; found melatonin plays a protective role in mitochondrial fission-VDAC1-HK2-mPTP-mitophagy axis signal pathway suppression. PMID: 28398674
    4. microcirculatory ischemia/reperfusion injury can be attributed to Mff-dependent mitochondrial fission via voltage-dependent anion channel 1/hexokinase 2-mediated mitochondrial permeability transition pore opening and mitochondrial reactive oxygen species/cardiolipin involved cyt-c release. PMID: 28288978
    5. CD4 T cell mediated immuno-inflammatory responses to a virus infection were similar between WT and HK2 KO animals. The observations that the expression of HK2 appears non-essential for CD4 T cell responses against virus infections is of interest since it suggests that targeting HK2 for cancer therapy may not have untoward effects on CD4 T cell mediated immune response against virus infections. PMID: 29352298
    6. BAG3 directly stabilizes hexokinase 2 mRNA and promotes aerobic glycolysis in pancreatic cancer cells. PMID: 29114069
    7. changes in mitochondrial HKII modestly affect cardiac oxygen consumption and energy substrate metabolism PMID: 28641785
    8. Hexokinase II (HKII) binding to the mitochondria is decreased in muscle from high fat diet-fed SIRT3 KO mice. PMID: 25948682
    9. HK2 is upregulated in prostate cancer cells harboring Pten/p53 mutations. HK2 is required for Pten-/p53-deficiency-driven prostate tumor growth in vivo. PMID: 25176644
    10. A lack of effect on gene expression, changes in the protein expression patterns of the key genes GLUT1/SLC2A1 and HK2 were observed after radiation treatment. PMID: 25304950
    11. Both HK2 mRNA and protein were increased under hypoxia, which is accompanied by an increase of glucose uptake and production of lactate. PMID: 25172909
    12. Suggest that HK2 attenuates cardiac hypertrophy by decreasing ROS accumulation via increased pentose phosphate pathway flux. PMID: 24190878
    13. phosphorylation-mediated regulation of mitochondrial HK-II would be a critical component of the protective effect of Akt. PMID: 23836898
    14. Reduced muscle HKII protein content results in impaired muscle functionality during recovery from ischemic-reperfusion injury. PMID: 22723631
    15. HKII mitochondrial binding is also important for the hypertrophic effects, as HKII dissociation from the mitochondria resulted in de novo hypertrophy. PMID: 22517678
    16. We identified miR-143 as an essential regulator of cancer glycolysis via targeting HK2. PMID: 22593586
    17. PPARgamma contributes to PKM2 and HK2 expression in fatty liver PMID: 22334075
    18. In a cardiac low-flow ischaemia-reperfusion model, resulting in only mild injury, standard chow or reduced HKII does not significantly affect ischaemia-reperfusion damage. PMID: 21504994
    19. UCP3 was required for the HKII-mediated decrease in mitochondrial ROS emission PMID: 21554247
    20. The association of hexokinase II with mitochondria is essential for the protective effects of ischemic preconditioning and normal cardiac function through maintenance of mitochondrial potential. PMID: 21527739
    21. reduction in HKII levels causes altered remodeling of the heart in I/R by increasing cell death and fibrosis and reducing angiogenesis and that mitochondrial binding is needed for protection of cardiomyocytes. PMID: 21071708
    22. Interleukin 6 enhances glycolysis through expression of the glycolytic enzymes hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3. PMID: 20453422
    23. The data suggest for the first time that HKII mediates skeletal muscle ischemia-reperfusion injury in the intact male animal. PMID: 20182739
    24. IL-7 controls glucose utilization by regulating the gene expression of HXKII, suggesting a mechanism by which IL-7 supports bioenergetics that control cell fate decisions in lymphocytes. PMID: 20200205
    25. induction of expression and activity by HB-EGF PMID: 11782486
    26. muscle glucose uptake is impaired by reductions in hexokinase II activity during exercise PMID: 12865258
    27. IL-1beta increased HK activity in both a time- and concentration-dependent manner in glomerular mesangium cells PMID: 15070811
    28. adaptations that increase Hexokinase II protein content and/or functional activity such as regular exercise contribute to increased muscular endurance PMID: 15878951
    29. IRS2, HK2 and INSIG1 are direct targets of TFE3 PMID: 16327801
    30. Impairments in whole-body metabolism and muscle glucose uptake due to high-fat feeding and partial HK II knockout mice PMID: 17639019
    31. hexokinase II gene inhibits human colon cancer LoVo cell growth PMID: 18535403
    32. mTORC1 hyperactivity contributes to the apoptotic resistance of serum-deprived Tsc1/2-deficient cells in part by increasing the growth factor-independent expression of hexokinase II (HKII) and GLUT1. PMID: 19620286

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  • 亞細(xì)胞定位：
    Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol.
  • 蛋白家族：
    Hexokinase family
  • 組織特異性：
    Predominantly expressed in skeletal muscle, heart and adipose tissues.
  • 數(shù)據(jù)庫(kù)鏈接：