E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Association between GAD1 rs3749034 polymorphisms with antisaccade task performance in schizophrenic patients. PMID: 29429137
This study detected significant reductions in the mRNAs associated with GAD1 in the frontal cortex (FC) of autism spectrum disorder. PMID: 28229923
Our results demonstrate a weak effect of the GAD1 gene on the risk of bipolar illness, and the associated marker might represent a proxy for real signals of rare variants. PMID: 28710909
findings converge on variation in glutamic acid decarboxylase 1 gene predicting a susceptibility mechanism that affects white matter fractional anisotropy, GABAergic inhibitory neurotransmission in the dorsolateral prefrontal cortex, and working memory performance. PMID: 26822489
Results show how epigenetic changes in GAD1 expression alter local glutamate metabolism in the brain metastatic microenvironment, contributing to a metabolic adaption that facilitates metastasis outgrowth in that setting. PMID: 28400476
We investigate the possible influence of GAD1 SNPs rs3749034 and rs11542313 on ADHD susceptibility. The C allele of rs11542313 was significantly overtransmitted from parents to ADHD probands. Hyperactive/impulsive score was higher in rs3749034G allele and rs11542313C allele carriers. GAD1 haplotypes were associated with higher hyperactive/impulsive scores in ADHD youths. GAD1 gene is associated with ADHD susceptibility. PMID: 27530595
GAD1 is reactivated by DNA methylation, which provided a model for DNA methylation and the active orchestration of oncogenic gene expression by CTCF in cancer cells. PMID: 26549033
There was no difference in GAD25 and GAD67 gene expression level, and GAD25/GAD67 ratio between patients with first episode psychosis and healthy controls PMID: 28122016
Genetic variability in GAD1 and GAD2 contributes to the risk of methamphetamine dependence in the Thai population. PMID: 27967329
Reduced GAD67 expression in PV neurons is not an upstream cause of the lower levels of GABA-associated transcripts, or of the characteristic behaviors, in schizophrenia. PMID: 26980143
Glutamic acid decarboxylase 67 (GAD67) is one of the isoforms that catalyze GABA synthesis. Here, we used recombinant herpes simplex virus (HSV-1) vectors that encode gad1 gene to evaluate the therapeutic potential of GAD67 in peripheral HIV gp120-induced neuropathic pain PMID: 26752351
This study showed that in female groups, the expression of GABRA5 was generally higher in schizophrenia cases compared to the control. PMID: 25660468
GAD autoantibodies were associated with risk of developing type 1 diabetes. PMID: 25523979
Conversion of GAD autoantibody is associated with patients with presumed type 2 diabetes. PMID: 25301693
The GAD65-independent mechanism for targeting of GAD67 to synaptic vesicles in neurons is not functional in islet beta-cells. PMID: 25647668
These results suggest a link between altered inhibitory mechanisms and synchrony and, therefore point to potential mechanisms via which a disruption in neurodevelopmental processes might lead to pathophysiology associated with schizophrenia. PMID: 25072323
Gene ransfer of GAD67 by herpes simplex virus vectors prevents HIV gp120/ddC-induced neuropathic pain. PMID: 25851180
Phosphate-activated glutaminase and GAD65/67 concentrations are compared in Alzheimer's disease cerebellum versus normal cerebellum controls PMID: 24950944
results statistically show that a reduction in GAD1 and SCL1A2 expression in the dorsolateral prefrontal cortex in subjects with major depressive disorder is related to a possible attenuated RAF/MEK/ERK pathway PMID: 24652383
CNR1, GAD1 and BDNF polymorphisms are associated with male heroin dependence in the Dai population in China. PMID: 25252306
The decreased amount of GAD65 and GAD67 suggests the decreased synthesis of neurotransmitter and basic GABA pools that indicates insufficient functioning of the GABA system in the cerebellar cortex of Alzheimer's disease patients. PMID: 24874321
Deficient Zif268 mRNA expression may contribute to lower cortical GAD67 levels in schizophrenia, suggesting altered cortical GABA synthesis and impaired cognition in schizophrenia. PMID: 24874453
A compensatory role was found for the GAD1 gene hypomethylation in panic disorder depending on genetic variation. PMID: 23906988
GAD1 expression correlates with cellular invasiveness and migration by regulating beta-catenin translocation and MMP7 activation. PMID: 24261884
The data of this study showed a robust reduction of cortical thickness in the left parahippocampal gyrus (PHG) in G allele homozygotes of GAD1 rs3749034. PMID: 23566421
The GG GAD1 haplotype (rs3791862, rs16858977) frequency demonstrated significantly statistical difference between cerebral palsy patients and controls. PMID: 24228494
Single nucleotide polymorphism(SNP)s in the GAD1 gene are associated with increased susceptibility to post-traumatic seizures after traumatic brain injury. PMID: 22840783
In schizophrenia, abnormal PSA-NCAM and GAD67 expression may underlie the alterations observed in inhibitory neurotransmission. PMID: 23022470
The present study represents the first systematic evaluation of gender-specific enrichment of risk alleles of the common SNP variation in the panic disorder candidate gene GAD1. PMID: 22662185
This study provides further support for involvement of mainly GAD1, but also NPY in determining predisposition to anxiety disorders. PMID: 22328461
Changes in copy number intensities vary in accordance with the disturbances in the expression of mRNAs associated with GAD67 regulation, but the patterns seen are fundamentally different for patients with schizophrenia and patients with bipolar disorder. PMID: 22309971
Anti-idiotypic antibodies specific to GAD65Ab have a protective role in type 1 diabetes PMID: 22384267
These findings point to a role for GAD1 polymorphism in heroin dependence among Han Chinese, and may be informative for future genetic or neurobiological studies on heroin dependence. PMID: 22564729
Glutamate decarboxylase 67 is expressed in hippocampal mossy fibers of temporal lobe epilepsy patients PMID: 21509853
The expression of synaptophysin and GAD67 was downregulated in the basolateral nucleus of schizophrenics. PMID: 22099865
From midgestation to infancy, the pattern of GABAA receptor binding changed from uniformly low across all cortical layers to high levels concentrated in the middle laminae;GAD67 levels differentially increased. PMID: 21937910
Support for the hypothesis that lower GABA synthesis in parvalbumin neurons contributes to dorsolateral prefrontal cortex dysfunction and impaired cognition in schizophrenia. PMID: 21632647
Our findings show abnormal BDNF, trkB-TK+ and GAD67 mRNA expression in the hippocampus of individuals with schizophrenia and mood disorders. PMID: 21223646
GAD65 is the major autoantigen in type 1 diabetes, and GAD67 per se has very low immunogenicity. PMID: 21494613
Alterations of glutamic acid decarboxylase (GAD) play a crucial role in schizophrenic pathology. Read More: http://informahealthcare.com/doi/full/10.3109/15622975.2010.539270 PMID: 21250934
Observations suggest that genetic variation in the GAD1 gene is unlikely to play a critical role in pervasive child development disorders. PMID: 21302352
The correlation of GAD67 gene expression with MUC5AC expressions suggests that the up-regulation of airway epithelium expression of GAD67 may contribute to the increase in mucus production observed in association with cigarette smoking. PMID: 21034448
some of Japanese patients who presented with acute onset diabetic ketosis and negative for GADAb share several clinical characteristics with atypical type 2 diabetes such as ketosis-prone diabetes and "soft-drink ketosis," but others do not PMID: 20960264
results highlight a statistical association between one SNP of GAD67 (rs 11542313) and the condition of alcohol dependence. PMID: 20598486
Analysis of expression data from a microarray study performed in samples from Brodmann area 9 confirms that GAD67 expression is decreased in Parkinson's disease PMID: 20832408
These results support the importance of genetic variation in GAD1 in regulating prefrontal cortical GABA function. PMID: 20357758
The overall amount of GAD-67 was significantly reduced (-34%) in depressed subjects compared to matched controls. PMID: 20236554
Subjects with schizophrenia showed a significant, on average 8-fold deficit in repressive chromatin-associated DNA methylation at the GAD1 promoter. PMID: 17726539
GAD gene transfer into glutamatergic excitatory neurons leading to an inhibitory bias with altered network activity and a neuroprotective phenotype holds potential for treatment of Parkinson's disease PMID: 12376704
autoreactivity to GAD25 is rare in newly diagnosed type 1 diabetes and does not underlie GAD67 reactivity PMID: 12515288
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相關疾病:
Cerebral palsy, spastic quadriplegic 1 (CPSQ1)
蛋白家族:
Group II decarboxylase family
組織特異性:
Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain.