Sheep ghrelin/obestatin prepropeptide (GHRL) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊饑餓激素(GHRL)酶聯(lián)免疫試劑盒（CSB-EL009414SH）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GHRL含量。靶點(diǎn)GHRL即胃饑餓素，它是一種主要由胃分泌的肽類激素。在研究機(jī)制方面，它與調(diào)節(jié)食欲、能量平衡及生長激素釋放等相關(guān)。其含量變化影響機(jī)體代謝，當(dāng)下圍繞它在肥胖、糖尿病等疾病中的作用及潛在治療價(jià)值展開諸多研究。試劑盒檢測范圍為62.5 pg/mL-4000 pg/mL，其應(yīng)用場景涵蓋綿羊營養(yǎng)代謝研究、能量穩(wěn)態(tài)相關(guān)疾病模型的分子機(jī)制解析，以及GHRL在胃腸道功能調(diào)控中的作用評(píng)估等科研領(lǐng)域。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

GHRL
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-4000 pg/mL
靈敏度：

15.6 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-98
1:2	Average %	97
	Range %	92-102
1:4	Average %	100
	Range %	94-106
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of sheep GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	95-105
EDTA plasma (n=4)	94	89-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.