Sheep adrenocorticotropic hormone (ACTH) ELISA kit

產(chǎn)品詳情

文獻(xiàn)引用 (2) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

綿羊促腎上腺皮質(zhì)激素(ACTH)酶聯(lián)免疫試劑盒（CSB-EQ027618SH）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的ACTH含量。促腎上腺皮質(zhì)激素（ACTH）是腺垂體分泌的多肽激素。其它對(duì)維持腎上腺正常形態(tài)和功能有重要作用。研究機(jī)制上，ACTH作用于腎上腺皮質(zhì)束狀帶，刺激糖皮質(zhì)激素合成與分泌，在應(yīng)激反應(yīng)等生理過(guò)程中發(fā)揮關(guān)鍵調(diào)節(jié)作用。試劑盒檢測(cè)范圍為0.097 pg/mL-400 pg/mL，適用于綿羊等反芻動(dòng)物應(yīng)激狀態(tài)評(píng)估、腎上腺功能研究、藥理實(shí)驗(yàn)中激素水平動(dòng)態(tài)監(jiān)測(cè)等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

ACTH
種屬：

Ovis aries (Sheep)
樣本類型：

serum, plasma
檢測(cè)范圍：

0.097 pg/mL-400 pg/mL
靈敏度：

0.097 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of sheep ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-89
1:2	Average %	91
	Range %	88-95
1:4	Average %	87
	Range %	83-95
1:8	Average %	100
	Range %	95-105

回收率：

The recovery of sheep ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-103
EDTA plasma (n=4)	89	87-92

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.