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Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

說明書下載

產品詳情

產品描述：

大鼠組織因子與活化凝血因子7復合物酶聯免疫試劑盒（CSB-EQ027252RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TF含量。TF即組織因子，是一種跨膜糖蛋白。它是外源性凝血途徑的啟動因子，在生理止血中起關鍵作用。研究發現TF還參與腫瘤生長、轉移及血管生成等。其作用機制主要是通過與凝血因子結合，啟動凝血級聯反應并激活相關信號通路。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于心血管疾病、血栓性疾病等動物模型的機制研究，或抗凝藥物、靶向治療方案的體外效果評估。為凝血相關基礎研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

TF
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	107
	Range %	98-110
1:2	Average %	103
	Range %	96-107
1:4	Average %	98
	Range %	89-102
1:8	Average %	105
	Range %	98-110

回收率：

The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	102	95-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.