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  4. Rat soluble fibrin monomer complex(SFMC)ELISA Kit

Rat soluble fibrin monomer complex(SFMC)ELISA Kit

  • 中文名稱：
    大鼠可溶性血纖蛋白單體復合物(SFMC)酶聯免疫試劑盒
  • 貨號：
    CSB-E16270r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠可溶性血纖蛋白單體復合物(SFMC)酶聯免疫試劑盒（CSB-E16270r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SFMC含量。試劑盒檢測范圍為62.5 ng/mL-4000 ng/mL，適用于血栓形成機制研究、心血管疾病動物模型評估、抗凝血藥物篩選等科研領域，特別在深靜脈血栓、彌散性血管內凝血（DIC）等病理模型的分子機制研究中具有重要應用價值。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    SFMC
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    62.5 ng/mL-4000 ng/mL
  • 靈敏度：
    15.6 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SFMC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:100 Average % 91  
    Range % 86-95  
    1:200 Average % 102  
    Range % 97-107  
    1:400 Average % 91  
    Range % 85-97  
    1:800 Average % 97  
    Range % 91-103  
  • 回收率：
    The recovery of rat SFMC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 89-98  
    EDTA plasma (n=4) 97 90-100  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    4000 2.655 2.598 2.627 2.433  
    2000 2.362 2.356 2.359 2.165  
    1000 1.907 1.963 1.935 1.741  
    500 1.153 1.178 1.166 0.972  
    250 0.835 0.832 0.834 0.640  
    125 0.462 0.498 0.480 0.286  
    62.5 0.334 0.339 0.337 0.143  
    0 0.193 0.195 0.194    
  • 數據處理：
  • 貨期：
    3-5 working days

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