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Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

  • 中文名稱:
    大鼠基質金屬蛋白酶1(MMP-1/Collagenase I)酶聯免疫試劑盒
  • 貨號:
    CSB-E07416r
  • 規格:
    96T/48T
  • 價格:
    ¥3600/¥2500
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    大鼠基質金屬蛋白酶1(MMP-1/Collagenase I)酶聯免疫試劑盒(CSB-E07416r)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的MMP1含量。MMP1,即基質金屬蛋白酶1,是一種鋅依賴性內肽酶,參與細胞外基質(ECM)的降解,與細胞遷移、炎癥和腫瘤侵襲等生理和病理過程密切相關。MMP1通過降解ECM中的膠原和蛋白聚糖,改變細胞微環境,促進腫瘤的侵襲和轉移。研究MMP1的機制有助于開發針對腫瘤侵襲和轉移的治療策略。試劑盒檢測范圍為0.235 ng/ml- 15 ng/ml,靈敏度為0.058 ng/ml。適用于炎癥模型、組織工程研究、纖維化疾病機制探索等科研場景,能夠為心血管疾病、關節炎等動物模型的分子機制研究提供可靠數據支持本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 別名:
    Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
  • 縮寫:
  • Uniprot No.:
  • 種屬:
    Rattus norvegicus (Rat)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    0.235 ng/ml - 15 ng/ml
  • 靈敏度:
    0.058 ng/ml
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Cancer
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase?I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

     

    Sample

    Serum(n=4)

    1:1

    Average %

    89

    Range %

    85-94

    1:2

    Average %

    92

    Range %

    87-98

    1:4

    Average %

    95

    Range %

    90-102

    1:8

    Average %

    100

    Range %

    96-109

  • 回收率:

    The recovery of rat MMP-1/Collagenase?I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    84

    80-89

    EDTA plasma (n=4)

    97

    90-104

  • 標準曲線:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    ng/ml

    OD1

    OD2

    Average

    Corrected

    15

    2.257

    2.231

    2.244

    2.147

    7.5

    1.876

    1.832

    1.854

    1.757

    3.75

    1.381

    1.373

    1.377

    1.280

    1.88

    0.835

    0.797

    0.816

    0.719

    0.94

    0.397

    0.376

    0.387

    0.290

    0.47

    0.273

    0.257

    0.265

    0.168

    0.235

    0.197

    0.207

    0.202

    0.105

    0

    0.099

    0.094

    0.097

     

  • 數據處理:
  • 貨期:
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 最新研究進展:
    MMP1,全名為基質金屬蛋白酶-1,是一種廣泛存在于人體組織中的酶類分子。MMP1主要參與膠原蛋白的降解和清除過程,在組織再生、炎癥、腫瘤轉移等多種生理和病理過程中發揮重要作用。最近的研究表明,MMP1在多種癌癥的發生和發展中發揮關鍵作用,成為了一個重要的治療靶點。此外,MMP1還與關節炎、動脈粥樣硬化等疾病的發生和發展密切相關,成為了重要的生物標志物和疾病預測因子。針對這些酶的抑制劑已成為研究的熱點,可以用于疾病的預防和治療。
  • 功能:
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
  • 亞細胞定位:
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族:
    Peptidase M10A family
  • 數據庫鏈接:

    UniGene: Rn.79007