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  4. Rat cyclophilin A,CyPA ELISA Kit

Rat cyclophilin A,CyPA ELISA Kit

  • 中文名稱：
    大鼠嗜環(huán)蛋白/親環(huán)素A(CyPA)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E14928r
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠嗜環(huán)蛋白/親環(huán)素A(CyPA)酶聯(lián)免疫試劑盒（CSB-E14928r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、細(xì)胞裂解物、組織勻漿樣本中的PPIA含量。PPIA即親環(huán)蛋白A，是一種廣泛存在于各類細(xì)胞中的蛋白質(zhì)。它參與眾多細(xì)胞活動，如免疫調(diào)節(jié)、炎癥反應(yīng)等。研究機制方面，其可通過肽基脯氨酰順反異構(gòu)酶活性影響蛋白質(zhì)折疊和功能，在疾病發(fā)生發(fā)展及藥物研發(fā)中是重要靶點。試劑盒檢測范圍為125 pg/mL-8000 pg/mL，適用于科研實驗中探索CyPA在疾病模型中的表達調(diào)控機制、炎癥相關(guān)信號通路研究以及藥物干預(yù)效果評估等場景，為體外實驗或動物模型中的生物標(biāo)志物分析提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Ppia ELISA Kit; Peptidyl-prolyl cis-trans isomerase A ELISA Kit; PPIase A ELISA Kit; EC 5.2.1.8 ELISA Kit; Cyclophilin A ELISA Kit; Cyclosporin A-binding protein ELISA Kit; Rotamase A ELISA Kit; p1B15 ELISA Kit; p31) [Cleaved into: Peptidyl-prolyl cis-trans isomerase A ELISA Kit; N-terminally processed] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell lysates, tissue homogenates
  • 檢測范圍：
    125 pg/mL-8000 pg/mL
  • 靈敏度：
    31.25 pg/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Signal Transduction
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CyPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:1Average %103
    Range %101-105
    1:2Average %96
    Range %93-98
    1:4Average %89
    Range %84-94
    1:8Average %98
    Range %96-100
  • 回收率：
    The recovery of rat CyPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 106103-109
    EDTA plasma (n=4)9286-98
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    80002.788 2.595 2.692 2.509
    40002.012 1.987 2.000 1.817
    20001.366 1.323 1.345 1.162
    10000.789 0.790 0.790 0.607
    5000.512 0.526 0.519 0.336
    2500.389 0.382 0.386 0.203
    1250.254 0.264 0.259 0.076
    00.180 0.186 0.183
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點詳情

  • 功能：
    Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Exerts a strong chemotactic effect on leukocytes partly through activation of one of its membrane receptors BSG/CD147, initiating a signaling cascade that culminates in MAPK/ERK activation. Activates endothelial cells (ECs) in a proinflammatory manner by stimulating activation of NF-kappa-B and ERK, JNK and p38 MAP-kinases and by inducing expression of adhesion molecules including SELE and VCAM1. Induces apoptosis in ECs by promoting the FOXO1-dependent expression of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis. In response to oxidative stress, initiates proapoptotic and antiapoptotic signaling in ECs via activation of NF-kappa-B and AKT1 and up-regulation of antiapoptotic protein BCL2. Negatively regulates MAP3K5/ASK1 kinase activity, autophosphorylation and oxidative stress-induced apoptosis mediated by MAP3K5/ASK1. Necessary for the assembly of TARDBP in heterogeneous nuclear ribonucleoprotein (hnRNP) complexes and regulates TARDBP binding to RNA UG repeats and TARDBP-dependent expression of HDAC6, ATG7 and VCP which are involved in clearance of protein aggregates. Plays an important role in platelet activation and aggregation. Regulates calcium mobilization and integrin ITGA2B:ITGB3 bidirectional signaling via increased ROS production as well as by facilitating the interaction between integrin and the cell cytoskeleton. Binds heparan sulfate glycosaminoglycans.
  • 亞細(xì)胞定位：
    Cytoplasm. Secreted. Nucleus.
  • 蛋白家族：
    Cyclophilin-type PPIase family, PPIase A subfamily
  • 數(shù)據(jù)庫鏈接：