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  4. Rat Serum amyloid A,SAA ELISA Kit

Rat Serum amyloid A,SAA ELISA Kit

  • 中文名稱：
    大鼠血清淀粉樣蛋白A(SAA)酶聯免疫試劑盒
  • 貨號：
    CSB-E08590r
  • 規格：
    96T/48T
  • 價格：
    ￥3900/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠血清淀粉樣蛋白A(SAA)酶聯免疫試劑盒（CSB-E08590r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SAA含量。試劑盒檢測范圍為78 ng/mL-5000 ng/mL，適用于炎癥機制研究、感染性疾病模型評估、免疫調節藥物開發等科研領域，例如在實驗性關節炎、膿毒癥或腫瘤模型中動態監測SAA水平變化，為病理機制解析及治療干預效果評價提供可靠數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    SAA
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    78 ng/mL-5000 ng/mL
  • 靈敏度：
    19.5 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cardiovascular
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %96
    Range %93-99
    1:2Average %94
    Range %91-97
    1:4Average %100
    Range %97-103
    1:8Average %102
    Range %99-105
  • 回收率：
    The recovery of rat SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 8782-91
    EDTA plasma (n=4)9895-101
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    50001.978 1.998 1.988 1.846
    25001.341 1.398 1.370 1.228
    12500.813 0.834 0.824 0.682
    6250.476 0.498 0.487 0.345
    312.50.308 0.328 0.318 0.176
    156.250.245 0.258 0.252 0.110
    780.202 0.215 0.209 0.067
    00.141 0.143 0.142 　
  • 數據處理：
  • 貨期：
    3-5 working days

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