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  4. Rat Plasma kallikrein(KLKB1) ELISA kit

Rat Plasma kallikrein(KLKB1) ELISA kit

  • 中文名稱：
    大鼠血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒
  • 貨號：
    CSB-EL012461RA
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒（CSB-EL012461RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KLKB1含量。KLKB1 是一個重要靶點。它參與多種生理病理過程，如凝血與纖溶系統調節。在研究機制上，其活性變化可影響血管通透性、炎癥反應等。對 KLKB1 的深入研究有助于揭示相關疾病發病機制，為開發針對性藥物和治療策略提供方向。試劑盒檢測范圍為7.8 ng/mL-500 ng/mL，適用于心血管疾病、代謝調控及炎癥相關機制的基礎研究，也可用于藥理學實驗中藥物對 KLKB1 表達水平影響的評估。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    7.8 ng/mL-500 ng/mL
  • 靈敏度：
    1.95 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:200Average %91
    Range %86-95
    1:400Average %103
    Range %99-105
    1:800Average %91
    Range %85-97
    1:1600Average %96
    Range %91-99
  • 回收率：
    The recovery of rat KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-98
    EDTA plasma (n=4)9795-99
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    5002.648 2.524 2.586 2.493
    2502.335 2.287 2.311 2.218
    1251.894 1.804 1.849 1.756
    62.51.112 1.125 1.119 1.026
    31.20.647 0.600 0.624 0.531
    15.60.370 0.352 0.361 0.268
    7.80.241 0.222 0.232 0.139
    00.091 0.095 0.093 　
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasma kallikrein subfamily
  • 數據庫鏈接：