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  4. Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

Rat N-acetyl-β-D-glucosaminidase,NAG ELISA Kit

  • 中文名稱：
    大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯免疫試劑盒
  • 貨號：
    CSB-E07443r
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠N-乙酰-β-D-氨基葡萄糖苷酶(NAG)酶聯免疫試劑盒（CSB-E07443r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養上清液、組織勻漿、尿液樣本中的NAG含量。大鼠N-乙酰-β-D-氨基葡萄糖苷酶（NAG）是一種溶酶體水解酶，主要存在于近端腎小管中，與黏多糖類及糖蛋白代謝相關。其活性變化與腎小管損傷密切相關，可作為腎損傷的早期標志物。目前，NAG的研究主要集中在其生物學機制、靶點以及與腎小管疾病的關系上。試劑盒檢測范圍為1.56 mIU/mL-100 mIU/mL，該產品可廣泛應用于腎臟疾病機制研究、藥物腎毒性評估、代謝綜合征模型構建等科研領域，尤其適用于動物實驗中對尿液或血液樣本的NAG動態監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Hexb ELISA Kit; Beta-hexosaminidase subunit beta ELISA Kit; EC 3.2.1.52 ELISA Kit; Beta-N-acetylhexosaminidase subunit beta ELISA Kit; Hexosaminidase subunit B ELISA Kit; N-acetyl-beta-glucosaminidase subunit beta ELISA Kit
  • 縮寫：
    NAG
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates, urine
  • 檢測范圍：
    1.56 mIU/mL-100 mIU/mL
  • 靈敏度：
    0.39 mIU/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Others
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat NAG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:1Average %95
    Range %90-100
    1:2Average %96
    Range %91-102
    1:4Average %87
    Range %84-95
    1:8Average %95
    Range %92-101
  • 回收率：
    The recovery of rat NAG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10095-105
    EDTA plasma (n=4)9690-102
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    mIU/mlOD1OD2AverageCorrected
    1002.495 2.471 2.483 2.306
    501.955 1.987 1.971 1.794
    251.356 1.368 1.362 1.185
    12.50.934 0.963 0.949 0.772
    6.250.587 0.597 0.592 0.415
    3.120.425 0.451 0.438 0.261
    1.560.361 0.374 0.368 0.191
    00.175 0.178 0.177 　
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

靶點詳情

  • 功能：
    Hydrolyzes the non-reducing end N-acetyl-D-hexosamine and/or sulfated N-acetyl-D-hexosamine of glycoconjugates, such as the oligosaccharide moieties from proteins and neutral glycolipids, or from certain mucopolysaccharides. The isozyme B does not hydrolyze each of these substrates, however hydrolyzes efficiently neutral oligosaccharide. Only the isozyme A is responsible for the degradation of GM2 gangliosides in the presence of GM2A. During fertilization is responsible, at least in part, for the zona block to polyspermy. Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and inactivates the sperm galactosyltransferase-binding site, accounting for the block in sperm binding to the zona pellucida.
  • 亞細胞定位：
    Lysosome. Cytoplasmic vesicle, secretory vesicle, Cortical granule.
  • 蛋白家族：
    Glycosyl hydrolase 20 family
  • 數據庫鏈接：

    KEGG: rno:294673

    UniGene: Rn.203067