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  4. Rat Interleukin 1α,IL-1α ELISA kit

Rat Interleukin 1α,IL-1α ELISA kit

  • 中文名稱：
    大鼠白介素1α(IL-1α)酶聯免疫試劑盒
  • 貨號：
    CSB-E04622r
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    大鼠白介素1α(IL-1α)酶聯免疫試劑盒（CSB-E04622r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿.樣本中的IL1A含量。IL1A 是一種細胞因子，在炎癥和免疫反應中扮演關鍵角色。其背景上，它與多種疾病如自身免疫病、炎癥性疾病相關。研究機制顯示，IL1A 可激活下游信號通路，引發機體免疫細胞反應和炎癥因子釋放，是潛在的疾病治療靶點。試劑盒檢測范圍為78 pg/ml-5000 pg/ml.，靈敏度為19.5 pg/ml.。適用于科研領域中對炎癥機制、免疫調控模型及藥物干預效果的評估，尤其適合同時分析體液樣本（血清/血漿）與組織局部IL - 1α表達水平。為研究IL - 1α在先天免疫應答、細胞應激反應及病理狀態下的動態變化提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates.
  • 檢測范圍：
    78 pg/ml-5000 pg/ml.
  • 靈敏度：
    19.5 pg/ml.
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess.

    Inter-assay Precision (Precision between assays): CV%<10%

    Three samples of known concentration were tested in twenty assays to assess.

  • 線性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-1α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    　

    Sample

    Serum(n=4)

    1:1

    Average %

    96

    Range %

    92-102

    1:2

    Average %

    98

    Range %

    91-104

    1:4

    Average %

    101

    Range %

    98-107

    1:8

    Average %

    89

    Range %

    84-96

  • 回收率：

    The recovery of rat IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range

    Serum (n=5)

    98

    92-106

    EDTA plasma (n=4)

    95

    89-99

  • 標準曲線：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

    pg/ml

    OD1

    OD2

    Average

    Corrected

    5000

    2.657

    2.556

    2.607

    2.425

    2500

    2.123

    2.022

    2.073

    1.891

    1250

    1.468

    1.447

    1.458

    1.276

    625

    0.927

    0.916

    0.922

    0.740

    312

    0.568

    0.579

    0.574

    0.392

    156

    0.415

    0.404

    0.410

    0.228

    78

    0.312

    0.300

    0.306

    0.124

    0

    0.180

    0.183

    0.182

    　

  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 基因功能參考文獻：
    1. We propose that induction of collagenase-1 by IL-1alpha in both WF and NF depends on a unique combination of cell type-specific signaling pathways. PMID: 17455222
  • 亞細胞定位：
    Cytoplasm. Secreted.
  • 蛋白家族：
    IL-1 family
  • 數據庫鏈接：