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  4. Rat Interleukin-17F(IL17F) ELISA kit

Rat Interleukin-17F(IL17F) ELISA kit

  • 中文名稱：
    大鼠白介素17F(IL17F)酶聯(lián)免疫試劑盒
  • 貨號(hào)：
    CSB-EL011601RA
  • 規(guī)格：
    96T/48T
  • 價(jià)格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    大鼠白介素17F(IL17F)酶聯(lián)免疫試劑盒（CSB-EL011601RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL17F含量。IL17F是重要靶點(diǎn)。它屬IL-17細(xì)胞因子家族，可調(diào)節(jié)免疫反應(yīng)。研究發(fā)現(xiàn)，其參與多種炎癥和自身免疫疾病的發(fā)病機(jī)制，過度表達(dá)會(huì)引發(fā)異常免疫。目前圍繞它開發(fā)拮抗劑等藥物，以阻斷其信號(hào)傳導(dǎo)治療相關(guān)疾病。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，本試劑盒適用于科研領(lǐng)域中對(duì)大鼠模型中IL - 17F表達(dá)水平的動(dòng)態(tài)監(jiān)測，如炎癥性疾病機(jī)制研究、免疫調(diào)節(jié)實(shí)驗(yàn)或藥物干預(yù)效果評(píng)估等場景，為探索IL - 17F在免疫系統(tǒng)中的功能提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    Il17fInterleukin-17F ELISA Kit; IL-17F ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Rattus norvegicus (Rat)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    15.6 pg/mL-1000 pg/mL
  • 靈敏度：
    3.9 pg/mL
  • 反應(yīng)時(shí)間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 91-99
    1:2 Average % 83
    Range % 80-86
    1:4 Average % 95
    Range % 80-90
    1:8 Average % 102
    Range % 98-106
  • 回收率：
    The recovery of rat IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 87 83-90
    EDTA plasma (n=4) 104 100-108
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    1000 2.806 2.769 2.788 2.693
    500 2.304 2.401 2.353 2.258
    250 1.535 1.605 1.570 1.475
    125 0.680 0.698 0.689 0.594
    62.5 0.343 0.351 0.347 0.252
    31.2 0.202 0.199 0.201 0.106
    15.6 0.127 0.129 0.128 0.033
    0 0.095 0.094 0.095 　
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 功能：
    Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity. IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation. IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation. As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites. Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers. IL17F homodimer can signal via IL17RC homodimeric receptor complex, triggering downstream activation of TRAF6 and NF-kappa-B signaling pathway. Via IL17RC induces transcriptional activation of IL33, a potent cytokine that stimulates group 2 innate lymphoid cells and adaptive T-helper 2 cells involved in pulmonary allergic response to fungi. Likely via IL17RC, promotes sympathetic innervation of peripheral organs by coordinating the communication between gamma-delta T cells and parenchymal cells. Stimulates sympathetic innervation of thermogenic adipose tissue by driving TGFB1 expression. Regulates the composition of intestinal microbiota and immune tolerance by inducing antimicrobial proteins that specifically control the growth of commensal Firmicutes and Bacteroidetes.
  • 基因功能參考文獻(xiàn)：
    1. Increased RORgammat and IL-17F levels contribute to Spontaneously Hypertensive Rat hypertension and might be therapeutic targets PMID: 28093217
    2. important mediator of increased endothelial permeability PMID: 19577259
  • 亞細(xì)胞定位：
    Secreted.
  • 蛋白家族：
    IL-17 family
  • 數(shù)據(jù)庫鏈接：