Rat Antithrombin III(AT III)ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

大鼠抗凝血酶III(AT III)酶聯(lián)免疫試劑盒（CSB-E13885r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的AT III含量。AT III（抗凝血酶III）是一種由肝臟和血管內(nèi)皮細胞合成的絲氨酸蛋白酶抑制蛋白，是人體內(nèi)重要的抗凝血因子，在生理性凝血機制中起關鍵作用。其研究機制主要涉及通過抑制凝血酶及多種凝血因子活性來防止血栓形成，臨床應用中主要用于治療血栓栓塞性疾病。試劑盒檢測范圍為39 ng/mL-2500 ng/mL，適用于各類科研實驗中AT III水平的精確分析，尤其適用于心血管疾病模型、藥物抗凝機制研究、肝臟功能評估等科研場景，為探索凝血 - 抗凝系統(tǒng)調(diào)控及病理生理機制提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AT III
Uniprot No.：

Q5M7T5
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

39 ng/mL-2500 ng/mL
靈敏度：

9.75 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat AT III in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	104
	Range %	100-112
1:400	Average %	84
	Range %	80-88
1:800	Average %	90
	Range %	85-94
1:1600	Average %	97
	Range %	92-101

回收率：

The recovery of rat AT III spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.