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  4. Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

Rabbit matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA Kit

  • 中文名稱：
    兔基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯(lián)免疫試劑盒
  • 貨號：
    CSB-E10373Rb
  • 規(guī)格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產(chǎn)品詳情

  • 產(chǎn)品描述：
    兔基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/Gelatinase A)酶聯(lián)免疫試劑盒（CSB-E10373Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的MMP2含量。MMP2 即基質(zhì)金屬蛋白酶 2，在多種生理和病理過程中起關(guān)鍵作用，如組織重塑、腫瘤轉(zhuǎn)移等。其研究機(jī)制主要圍繞它對細(xì)胞外基質(zhì)的降解功能，可破壞基底膜促使腫瘤細(xì)胞遷移。深入研究 MMP2 有助于揭示疾病發(fā)生發(fā)展機(jī)制，為相關(guān)疾病治療提供靶點(diǎn)。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，支持科研領(lǐng)域?qū)游锛膊∧Ｐ停ㄈ珀P(guān)節(jié)炎、腫瘤移植模型）的分子機(jī)制研究，或用于細(xì)胞培養(yǎng)上清、組織提取液中MMP - 2動態(tài)變化的分析，為探索基質(zhì)代謝調(diào)控、藥物干預(yù)效果評估提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
  • 別名：
    MMP2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Oryctolagus cuniculus (Rabbit)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    1.56 ng/mL-100 ng/mL
  • 靈敏度：
    0.39 ng/mL
  • 反應(yīng)時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領(lǐng)域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:10Average %89
    Range %85-95
    1:20Average %96
    Range %90-105
    1:40Average %93
    Range %85-99
    1:80Average %94
    Range %86-98
  • 回收率：
    The recovery of rabbit MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9589-103
    EDTA plasma (n=4)9287-100
  • 標(biāo)準(zhǔn)曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/mlOD1OD2AverageCorrected
    1002.506 2.531 2.519 2.419
    502.048 2.005 2.027 1.927
    251.674 1.691 1.683 1.583
    12.51.184 1.207 1.196 1.096
    6.250.882 0.879 0.881 0.781
    3.120.554 0.543 0.549 0.449
    1.560.306 0.314 0.310 0.210
    00.102 0.097 0.100
  • 數(shù)據(jù)處理：
  • 貨期：
    3-5 working days

產(chǎn)品評價

靶點(diǎn)詳情

  • 功能：
    Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues.; PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels.
  • 基因功能參考文獻(xiàn)：
    1. These results show that MMP-2 activates the EGFR and triggers downstream signaling pathways increasing Reactive Oxygen Species formation and promoting vasoconstriction. PMID: 30029165
    2. The present study demonstrated the ability of 30 and 100 ng/ml TIMP3 to attenuate migration and proliferation, and to inhibit the activity of MMP2, MMP9 and TNFalpha secretion of NA SMCs. In conclusion, TIMP3 may be considered a potential therapeutic drug for use in a novel drugeluting stent, to attenuate the progressive dilation of the aortic NA. PMID: 29956789
    3. Selenium suppressed high-fat diet-induced MMP2 over-expression in vivo by improving lipid metabolism. PMID: 28595793
    4. Repetitive mechanical stimulation of tendon cells results in a small increase in matrix metalloproteinase2 levels, but it appears unlikely that serum matrix metalloproteinase2 will be a useful indicator of tendon overuse injury. PMID: 26909661
    5. Inflammatory factors such as TNF-alpha can stimulate MMP-2/9 activity in corneal epithelium cells. This may be a potential manipulating mechanism of MMP expression in the pathogenesis of corneal diseases PMID: 26125840
    6. Results provide evidence that MMP-2 bears the potentiality to cleave alpha-DG enriched from rabbit skeletal muscle indicating that this degradation indeed might also occur in vivo. PMID: 25483986
    7. These data suggest that OxyHb suppresses K V currents through both reactive oxygen species-dependent and independent pathways involving MMP activation. PMID: 25366605
    8. In conclusion, MMP-2 could be responsible for the proteolysis of dystrophin. PMID: 24791785
    9. Castor oil polymer induces bone formation with high matrix metalloproteinase-2 expression. PMID: 23670892
    10. MMP2 spinal cord expression is increased in cervical spondylotic myelopathy. PMID: 22673181
    11. Ulinastatin effectively inhibited the increased expression of MMP-2, MMP-3, and iNOS in degenerated NP cells induced by IL-1beta in vitro. PMID: 22853554
    12. Hemoperfusion could obviously reduce oxidative stress and the expression levels of MMP-2, MMP-9 and TIMP-1 in rabbits with acute paraquat poisoning. PMID: 22801080
    13. Exposure to long periods of light irrespective of its characteristics leads to the increased expression of some matrix metalloprpteases. PMID: 20238014
    14. The RNA interference targeting COX-2 can effectively inhibit the expression of COX-2 and MMP-2 in IL-1alpha stimulated rabbit corneal stromal cells in vitro. PMID: 19175164
    15. Our results strongly suggest that ischaemic postconditioning may exert part of its cardioprotective effects through the inhibition of MMP-2 activity. PMID: 19880538

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  • 亞細(xì)胞定位：
    Secreted, extracellular space, extracellular matrix. Membrane. Nucleus.
  • 蛋白家族：
    Peptidase M10A family
  • 數(shù)據(jù)庫鏈接：