Rabbit intercellular adhesion molecule 1,ICAM-1/CD54 ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子細胞間粘附分子1(ICAM-1/CD54)酶聯(lián)免疫試劑盒（CSB-E06974Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ICAM-1/CD54含量。ICAM-1/CD54是重要細胞黏附分子。其背景為在多種細胞表面表達，參與免疫應(yīng)答、炎癥反應(yīng)等生理病理過程。研究機制上，它能介導細胞間黏附與信號傳導，異常表達與多種疾病如炎癥性疾病、腫瘤轉(zhuǎn)移等密切相關(guān)，是潛在治療靶點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，兔類動物模型中炎癥相關(guān)疾病機制研究、免疫調(diào)節(jié)藥物開發(fā)評估、感染病理進程分析、心血管疾病模型、自身免疫性疾病研究、病毒/細菌感染實驗中的生物標志物動態(tài)監(jiān)測本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

ICAM-1/CD54
Uniprot No.：

U3KPG6
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	94
	Range %	91-98
1:10	Average %	99
	Range %	91-106
1:20	Average %	87
	Range %	82-94
1:40	Average %	94
	Range %	86-98

回收率：

The recovery of rabbit ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	100	96-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.