Rabbit Interleukin 18,IL-18 ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子白介素18(IL-18)酶聯(lián)免疫試劑盒（CSB-E13942Rb）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的IL-18含量。IL-18是一個(gè)重要靶點(diǎn)。它屬于白細(xì)胞介素家族，在免疫調(diào)節(jié)中發(fā)揮關(guān)鍵作用。研究發(fā)現(xiàn)，IL-18參與多種炎癥相關(guān)疾病發(fā)生發(fā)展。其作用機(jī)制主要是通過結(jié)合受體激活下游信號(hào)通路，調(diào)控免疫細(xì)胞功能，為相關(guān)疾病治療提供了新方向。試劑盒檢測(cè)范圍為12.5 pg/mL-800 pg/mL，適用于科研場(chǎng)景中IL - 18濃度的精準(zhǔn)分析，例如免疫機(jī)制研究、炎癥動(dòng)物模型評(píng)估或疫苗開發(fā)中免疫原性評(píng)價(jià)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-18
Uniprot No.：

B1A3U4
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測(cè)范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rabbit IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	102	92-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.