Rabbit Immunoglobulin A,IgA ELISA Kit

產(chǎn)品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

兔子免疫球蛋白A(IgA)酶聯(lián)免疫試劑盒（CSB-E06946Rb）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IgA含量。IgA即免疫球蛋白A，是人體黏膜免疫的主要抗體，在抵御病原體入侵、維持黏膜穩(wěn)態(tài)等方面有重要作用。其研究機制主要聚焦于IgA與抗原的識別結(jié)合、參與免疫調(diào)節(jié)過程，以及在黏膜相關(guān)疾病如感染性疾病、自身免疫病中的作用機制。試劑盒檢測范圍為12 ng/mL-3000 ng/mL，尤其適用于疫苗研究、腸道菌群互作實驗或慢性炎癥模型中黏膜免疫效能的評估，也可用于體外細(xì)胞實驗探究免疫調(diào)節(jié)因子對IgA分泌的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IgA
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

12 ng/mL-3000 ng/mL
靈敏度：

3 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	89
	Range %	84-97
1:2000	Average %	91
	Range %	89-96
1:4000	Average %	93
	Range %	87-104
1:8000	Average %	92
	Range %	86-98

回收率：

The recovery of rabbit IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	88-103
EDTA plasma (n=4)	95	85-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.