Rabbit Collagen Type Ⅱ,Col Ⅱ ELISA Kit

產品詳情

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文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔子Ⅱ型膠原(Col Ⅱ)酶聯免疫試劑盒（CSB-E06856Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的Col 含量。試劑盒檢測范圍為6.25 ng/mL-400 ng/mL，適用于科研場景中軟骨相關疾病模型研究，如關節炎病理機制探索、軟骨損傷修復評估或藥物干預效果驗證等領域，為組織工程、生物材料開發及退行性疾病研究提供可靠的檢測工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II
縮寫：

Col
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

6.25 ng/mL-400 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	90-102
1:2	Average %	107
	Range %	97-111
1:4	Average %	88
	Range %	82-92
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of rabbit Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-94
EDTA plasma (n=4)	102	92-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.