Pig hemoglobin (Hb) ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述:豬血紅蛋白(Hb)酶聯(lián)免疫試劑盒(CSB-EQ027276PI)為競爭法ELISA試劑盒,定量檢測血清、血漿、RBC裂解物.樣本中的Hb含量。Hb即血紅蛋白,是紅細(xì)胞內(nèi)運(yùn)輸氧的特殊蛋白質(zhì)。其與氧氣結(jié)合和釋放對維持機(jī)體氧供至關(guān)重要。在研究機(jī)制上,常關(guān)注其結(jié)構(gòu)與功能改變,如與疾病相關(guān)的變異影響攜氧能力,也有基于它開發(fā)藥物改善氧運(yùn)輸?shù)确矫娴难芯俊T噭┖袡z測范圍為0.625 μg/mL-40 μg/mL,適用于科研領(lǐng)域評估紅細(xì)胞功能及血紅蛋白代謝相關(guān)研究;可用于營養(yǎng)學(xué)研究中評估飼料中鐵元素吸收效率、生理學(xué)實(shí)驗(yàn)中的貧血模型構(gòu)建,或毒理學(xué)實(shí)驗(yàn)中紅細(xì)胞損傷程度的分析,為實(shí)驗(yàn)室提供穩(wěn)定、高效的檢測方案,滿足多種樣本類型的血紅蛋白定量需求。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫:Hb
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種屬:Sus scrofa (Pig)
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樣本類型:serum, plasma, lysate for RBC.
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檢測范圍:0.625 μg/mL-40 μg/mL
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靈敏度:0.312 μg/mL
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反應(yīng)時(shí)間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領(lǐng)域:Cardiovascular
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測定原理:quantitative
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測定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of pig Hb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 87-105 1:2 Average % 92 Range % 86-100 1:4 Average % 95 Range % 89-99 1:8 Average % 95 Range % 87-105 -
回收率:
The recovery of pig Hb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-99 Plasma (n=5) 96 90-102 -
標(biāo)準(zhǔn)曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average 40 0.181 0.179 0.180 20 0.270 0.263 0.267 10 0.472 0.477 0.475 5 0.722 0.738 0.730 2.5 1.425 1.412 1.419 1.25 2.073 2.051 2.062 0.625 2.525 2.563 2.544 0 2.743 2.775 2.759 -
數(shù)據(jù)處理:
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貨期:3-5 working days
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