Pig glial fibrillary acidic protein,GFAP ELISA Kit

產品詳情

<source id="swu2s"></source>

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬神經膠質纖維酸性蛋白(GFAP)酶聯免疫試劑盒（CSB-E14950p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GFAP含量。GFAP（膠質纖維酸性蛋白）是一種特定于中樞神經系統的III類中間絲蛋白，主要在星形膠質細胞中表達。其研究機制涉及基因表達調控、蛋白結構、功能以及與多種神經系統疾病的關系，如阿爾茨海默病和亞歷山大病。GFAP在維持星形膠質細胞形態、血腦屏障形成和突觸功能中發揮重要作用。試劑盒檢測范圍為0.1 ng/mL-20 ng/mL，本試劑盒適用于豬模型在神經退行性疾病研究（如阿爾茨海默病、帕金森病）、腦創傷模型評估、神經毒性實驗或神經炎癥機制探索等科研場景中，定量分析血液或組織樣本的GFAP動態變化，為神經病理學研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

GFAP
Uniprot No.：

F1RR02
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.1 ng/mL-20 ng/mL
靈敏度：

0.05 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	96
	Range %	92-99
1:4	Average %	92
	Range %	89-96
1:8	Average %	96
	Range %	90-102

回收率：

The recovery of pig GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-105
EDTA plasma (n=4)	96	92-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.