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Pig Soluble protein-100B,S-100B ELISA Kit

  • 中文名稱:
    豬S100B蛋白(S-100B)酶聯免疫試劑盒
  • 貨號:
    CSB-E14063p
  • 規格:
    96T/48T
  • 價格:
    ¥4200/¥3000
  • 促銷:
  • 其他:

產品詳情

  • 產品描述:
    豬S100B蛋白(S-100B)酶聯免疫試劑盒(CSB-E14063p)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的S-100B含量。S-100B是一種鈣結合蛋白,主要存在于神經膠質細胞中。其背景上與神經系統損傷、疾病相關。研究機制方面,它可作為生物標志物反映中樞神經系統損傷程度,還參與細胞增殖、分化等過程,在神經病理生理活動中發揮重要作用。試劑盒檢測范圍為78 pg/mL-5000 pg/mL,本試劑盒通過高特異性抗體對目標蛋白進行捕獲與檢測,可穩定應用于神經損傷模型研究、疾病機制探索或藥物干預效果評價等科研場景,為分析豬體內S100B蛋白的動態變化提供可靠工具。樣本兼容性強,支持直接檢測未經稀釋的體液樣本或經適當處理的組織勻漿,操作流程標準化,滿足實驗室對神經生物學、獸醫學或比較醫學等領域的研究需求。本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
  • 縮寫:
    S-100B
  • Uniprot No.:
  • 種屬:
    Sus scrofa (Pig)
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測范圍:
    78 pg/mL-5000 pg/mL
  • 靈敏度:
    19.5 pg/mL
  • 反應時間:
    1-5h
  • 樣本體積:
    50-100ul
  • 檢測波長:
    450 nm
  • 研究領域:
    Signal Transduction
  • 測定原理:
    quantitative
  • 測定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 線性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
     SampleSerum(n=4)
    1:1Average %95
    Range %91-100
    1:2Average %93
    Range %85-101
    1:4Average %86
    Range %80-98
    1:8Average %94
    Range %92-105
  • 回收率:
    The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 9583-102
    EDTA plasma (n=4)9592-100
  • 標準曲線:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    50002.025 2.067 2.046 1.887
    25001.574 1.524 1.549 1.390
    12501.183 1.169 1.176 1.017
    6250.821 0.812 0.817 0.658
    3120.567 0.534 0.551 0.392
    1560.354 0.373 0.364 0.205
    780.250 0.262 0.256 0.097
    00.164 0.153 0.159  
  • 數據處理:
  • 貨期:
    3-5 working days

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