Pig Interleukin 22(IL-22) ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

豬白介素22(IL-22)酶聯(lián)免疫試劑盒（CSB-E15847p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IL-22含量。IL-22是重要靶點(diǎn)。它是一種細(xì)胞因子，參與免疫調(diào)節(jié)。其背景在于在多種疾病如炎癥性腸病、皮膚病等中表達(dá)異常。研究機(jī)制方面，主要通過與受體結(jié)合，激活下游信號(hào)通路，影響細(xì)胞增殖、分化及炎癥反應(yīng)，為相關(guān)疾病治療提供方向。試劑盒檢測(cè)范圍為78 pg/mL-5000 pg/mL，適用于動(dòng)物免疫調(diào)控研究、病原感染后細(xì)胞因子表達(dá)分析、腸道屏障功能評(píng)估等科研方向，為探索豬類疾病模型、疫苗效果評(píng)價(jià)及營養(yǎng)干預(yù)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-22
Uniprot No.：

F1SH68
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

78 pg/mL-5000 pg/mL
靈敏度：

19.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	94-104
1:2	Average %	96
	Range %	89-100
1:4	Average %	95
	Range %	89-98
1:8	Average %	103
	Range %	95-107

回收率：

The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	91-103
EDTA plasma (n=4)	101	95-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.