Pig Interferon α,IFN-α ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

豬α干擾素(IFN-α)酶聯(lián)免疫試劑盒（CSB-E07328p）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的IFNA1含量。IFNA1 為干擾素α1，是 I 型干擾素家族成員。其背景上在抗病毒、免疫調(diào)節(jié)等方面有重要作用。研究機制表明它能與細(xì)胞表面受體結(jié)合，激活下游信號通路，誘導(dǎo)多種抗病毒蛋白產(chǎn)生，從而抑制病毒復(fù)制、調(diào)節(jié)免疫細(xì)胞活性。試劑盒檢測范圍為7.8 pg/mL-500 pg/mL，適用于體外實驗研究，如病毒感染模型中的細(xì)胞因子動態(tài)分析、免疫調(diào)節(jié)劑效果評估或疫苗免疫原性研究；動態(tài)監(jiān)測對研究豬類病毒感染機制、免疫應(yīng)答評估及疫苗研發(fā)具有重要意義。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IFN-α
Uniprot No.：

Q6VAB8
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, cell culture supernates, tissue homogenates
檢測范圍：

7.8 pg/mL-500 pg/mL
靈敏度：

1.95 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-99
1:2	Average %	98
	Range %	91-105
1:4	Average %	96
	Range %	92-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of pig IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	97	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.