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  4. Pig Immunoglobulin E,IgE ELISA Kit

Pig Immunoglobulin E,IgE ELISA Kit

  • 中文名稱：
    豬免疫球蛋白E(IgE)酶聯免疫試劑盒
  • 貨號：
    CSB-E06803p
  • 規格：
    96T/48T
  • 價格：
    ￥4200/￥3000
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    豬免疫球蛋白E(IgE)酶聯免疫試劑盒（CSB-E06803p）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的IgE含量。試劑盒檢測范圍為0.049 ng/mL-12.5 ng/mL，為研究豬類過敏性疾病機制、免疫調控及疫苗效果評估提供重要依據；適用于科研場景中探究豬群免疫狀態、環境致敏原篩查、飼料過敏性評估等研究，為動物免疫學及獸醫學研究提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 縮寫：
    IgE
  • 種屬：
    Sus scrofa (Pig)
  • 樣本類型：
    serum, plasma
  • 檢測范圍：
    0.049 ng/mL-12.5 ng/mL
  • 靈敏度：
    0.049 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Immunology
  • 測定原理：
    quantitative
  • 測定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 92  
    Range % 88-95  
    1:400 Average % 93  
    Range % 90-97  
    1:800 Average % 88  
    Range % 85-95  
    1:1600 Average % 95  
    Range % 91-101  
  • 回收率：
    The recovery of pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-98  
    EDTA plasma (n=4) 103 98-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    12.5 0.198 0.209 0.204    
    3.125 0.436 0.465 0.451    
    0.78 0.977 0.918 0.948    
    0.195 1.427 1.422 1.425    
    0.049 2.119 2.111 2.115    
    0 2.524 2.653 2.589    
  • 數據處理：
  • 貨期：
    3-5 working days

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