Pig Cystatin C,Cys-C ELISA Kit

產品詳情

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文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豬半胱氨酸蛋白酶抑制劑/胱抑素C(Cys-C)酶聯免疫試劑盒（CSB-E13282p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CST3含量。CST3即半胱氨酸蛋白酶抑制劑C，在人體中廣泛表達。它可抑制半胱氨酸蛋白酶的活性。在科研領域，其與神經系統疾病、腎臟疾病等方面研究較多，比如在神經退行性疾病中，CST3水平變化可能參與疾病發生發展機制。試劑盒檢測范圍為0.78 μg/mL-50 μg/mL，支持科研人員開展豬模型相關研究，如腎臟功能評估、代謝性疾病機制探索或藥物干預效果驗證等方向，尤其適用于動物實驗樣本中Cys-C表達水平的動態監測，為畜牧獸醫學、比較醫學及基礎病理學研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

CST3
Uniprot No.：

Q0Z8R0
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 μg/mL-50 μg/mL
靈敏度：

0.2 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig Cys-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	93
	Range %	89-97
1:4	Average %	92
	Range %	84-96
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of pig Cys-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	9-103
EDTA plasma (n=4)	93	89-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.