Mouse very low density lipoprotein(VLDL)ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 評(píng)價(jià) (1) 說(shuō)明書(shū)下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠極低密度脂蛋白(VLDL)酶聯(lián)免疫試劑盒（CSB-E17089m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VLDL含量。VLDL即極低密度脂蛋白，在人體脂質(zhì)代謝中發(fā)揮重要作用。它由肝臟合成并分泌，能運(yùn)輸內(nèi)源性甘油三酯至外周組織。研究發(fā)現(xiàn)，其代謝異常與動(dòng)脈粥樣硬化等心血管疾病密切相關(guān)，深入探究VLDL代謝機(jī)制對(duì)相關(guān)疾病防治意義重大。試劑盒檢測(cè)范圍為0.78 ng/mL-50 ng/mL，適用于代謝機(jī)制研究、藥物干預(yù)效果評(píng)估或營(yíng)養(yǎng)學(xué)模型中脂質(zhì)代謝動(dòng)態(tài)監(jiān)測(cè)，為心血管疾病或代謝綜合征基礎(chǔ)研究提供高效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
別名：

N/A
縮寫(xiě)：

VLDL
種屬：

Mus musculus (Mouse)
樣本類(lèi)型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

0.78 ng/mL-50 ng/mL
靈敏度：

0.195 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Cardiovascular
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse VLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	92
	Range %	86-98
1:200	Average %	99
	Range %	92-106
1:400	Average %	83
	Range %	80-85
1:800	Average %	89
	Range %	83-97

回收率：

The recovery of mouse VLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	91-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

5.0分 - 1 個(gè)評(píng)價(jià)

樣品類(lèi)型：組織勻漿液肝臟

樣品信息：小鼠

稀釋比：其他 1：5000

產(chǎn)品評(píng)價(jià)：我用 CSB-E17089m檢測(cè)正常和基因敲除小鼠肝臟VLDL含量，其OD值如圖片所示。預(yù)期結(jié)果和最終檢測(cè)結(jié)果類(lèi)似，較為準(zhǔn)確。

By 唐老師