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  4. Mouse plasma kallikrein(KLKB1) ELISA Kit

Mouse plasma kallikrein(KLKB1) ELISA Kit

  • 中文名稱：
    小鼠血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒
  • 貨號：
    CSB-E16637m
  • 規格：
    96T/48T
  • 價格：
    ￥3600/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠血漿激肽釋放酶(KLKB1)酶聯免疫試劑盒（CSB-E16637m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的KLKB1含量。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，支持基礎科研中KLKB1相關機制研究，例如炎癥模型中的激肽系統動態分析、藥物干預對凝血 - 纖溶平衡的影響評估，或代謝性疾病動物模型中酶活性變化的探索。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
  • 縮寫：
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, tissue homogenates
  • 檢測范圍：
    3.12 ng/mL-200 ng/mL
  • 靈敏度：
    0.78 ng/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cell Biology
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 99  
    Range % 96-102  
    1:2000 Average % 103  
    Range % 100-106  
    1:4000 Average % 89  
    Range % 84-94  
    1:8000 Average % 96  
    Range % 94-98  
  • 回收率：
    The recovery of mouse KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 93 89-97  
    EDTA plasma (n=4) 103 100-105  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    200 2.459 2.555 2.507 2.388  
    100 1.877 1.966 1.922 1.803  
    50 1.355 1.332 1.344 1.225  
    25 0.789 0.768 0.779 0.660  
    12.5 0.546 0.588 0.567 0.448  
    6.25 0.323 0.345 0.334 0.215  
    3.12 0.204 0.221 0.213 0.094  
    0 0.118 0.119 0.119    
  • 數據處理：
  • 貨期：
    3-5 working days

產品評價

靶點詳情

  • 功能：
    The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
  • 基因功能參考文獻：
    1. Plasma kallikrein mediates brain hemorrhage and edema caused by tissue plasminogen activator therapy in mice after stroke. PMID: 28130211
    2. we found that post-ejaculated semen from fertile wild-type males was solidified and the sperm were entrapped in Wnt7aCre/+;Esr1f/f uteri, compared to the watery semen (liquefied) found in Esr1f/f controls.Kallikrein-related peptidases 3 (KLK3) and other kallikrein-related peptidases from male prostate glands are responsible for semen liquefaction by cleaving gel-forming proteins PMID: 28414719
    3. Plasma kallikrein deletion prevents occlusive thrombus formation in mice with a minimal role in provoked bleeding. PMID: 22398951
    4. PKK or fXII deficiency reduced thrombus formation in both arterial and venous thrombosis models, without an apparent effect on hemostasis. PMID: 21821705
    5. the prekallikrein gene (Klkb1) is expressed highly in the mammary gland during stromal remodeling periods including puberty and postlactational involution. PMID: 19297327

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  • 亞細胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Plasma kallikrein subfamily
  • 數據庫鏈接：