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  4. Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

Mouse matrix metalloproteinase 1,MMP-1 ELISA kit

  • 中文名稱：
    小鼠基質金屬蛋白酶1(MMP-1)酶聯免疫試劑盒
  • 貨號：
    CSB-E07417m
  • 規格：
    96T/48T
  • 價格：
    ￥3200/￥2500
  • 促銷：
  • 其他：

產品詳情

  • 產品描述：
    小鼠基質金屬蛋白酶1(MMP-1)酶聯免疫試劑盒（CSB-E07417m）檢測血清、血漿、組織培養上清液、組織勻漿樣本中的MMP-1含量。MMP-1，也稱為間質膠原酶和成纖維細胞膠原酶，是基質金屬蛋白酶家族的一員。它參與細胞外基質的降解，在正常生理過程和疾病過程中發揮作用。MMP-1通過降解膠原蛋白和明膠等成分，影響細胞微環境，與腫瘤侵襲、轉移和骨關節炎等疾病相關。研究MMP-1有助于開發針對相關疾病的治療策略。試劑盒檢測范圍為47 pg/mL-3000 pg/mL，適用于體外實驗研究，可支持細胞功能探索、疾病模型建立及藥物作用機制分析等科研場景本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
  • 別名：
    Mmp1a ELISA Kit; McolAInterstitial collagenase A ELISA Kit; EC 3.4.24.7 ELISA Kit; Matrix metalloproteinase-1a ELISA Kit; MMP-1a ELISA Kit; Mcol-A ELISA Kit
  • 縮寫：
    MMP-1
  • Uniprot No.：
  • 種屬：
    Mus musculus (Mouse)
  • 樣本類型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 檢測范圍：
    47 pg/mL-3000 pg/mL
  • 靈敏度：
    11.75 pg/mL
  • 反應時間：
    1-5h
  • 樣本體積：
    50-100ul
  • 檢測波長：
    450 nm
  • 研究領域：
    Cancer
  • 測定原理：
    quantitative
  • 測定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 線性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:20 Average % 91  
    Range % 86-95  
    1:40 Average % 100  
    Range % 97-103  
    1:80 Average % 91  
    Range % 87-95  
    1:160 Average % 97  
    Range % 93-100  
  • 回收率：
    The recovery of mouse MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 96 90-98  
                 
                 
  • 標準曲線：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    3000 2.482 2.510 2.496 2.317  
    1500 1.855 1.786 1.821 1.642  
    750 1.246 1.232 1.239 1.060  
    375 0.748 0.774 0.761 0.582  
    187.5 0.495 0.456 0.476 0.297  
    93.75 0.322 0.339 0.331 0.152  
    46.88 0.253 0.242 0.248 0.069  
    0 0.180 0.178 0.179 　  
  • 數據處理：
  • 貨期：
    3-5 working days

引用文獻

產品評價

平均分:
5.0分 - 1 個評價

樣品類型：血清

樣品信息：小鼠

稀釋比：其他 1:10

產品評價： 我用CSB-E07417m檢測小鼠血清MMP-1水平，其OD如圖所示，試劑盒樣本充足，使用說明簡潔明了，操作容易上手，有助于實驗進展，滿意。

By 劉老師

靶點詳情

  • 功能：
    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. Able to degrade synthetic peptides and type I and II fibrillar collagen.
  • 基因功能參考文獻：
    1. The difficulty to effectively maintain Mmp1a in the zymogen form may account for the tight control of Mmp1a expression and reduced expression in normal tissue as compared to inflammatory states or cancer. PMID: 24737602
    2. Stromal Mmp1a drives in vivo tumorigenesis. PMID: 23708660
    3. Matrix metalloproteinase Mmp-1a is not required for growth and fertility in mice and has a role in lung cancer progression by modulating inflammatory responses PMID: 23548910
  • 亞細胞定位：
    Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Peptidase M10A family
  • 數據庫鏈接：