Mouse antidiuretic hormone/vasopressin/arginine vasopressin,ADH/VP/AVP ELISA Kit

產(chǎn)品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠抗利尿激素/血管加壓素/精氨酸加壓素(ADH/VP/AVP)酶聯(lián)免疫試劑盒（CSB-E09272m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液樣本中的AVP含量。AVP即精氨酸加壓素，它在人體生理中作用關鍵，可調(diào)節(jié)水鹽平衡、影響血管張力等。在研究機制方面，科研聚焦其受體亞型，探索通過作用于這些受體調(diào)控相關生理過程，有望為治療尿崩癥、心血管疾病等提供新策略。試劑盒檢測范圍為1.25 pg/mL-80 pg/mL，常用于內(nèi)分泌機制、水鹽代謝研究及高血壓或尿崩癥動物模型的科研分析；適用于實驗室對神經(jīng)內(nèi)分泌調(diào)節(jié)機制、病理生理模型中的激素動態(tài)變化以及藥物干預效應等科研場景的定量研究本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

ADH, ARVP, AVP-NPII, AVRP, VP, antidiuretic hormone|arginine vasopressin-neurophysin II|neurohypophyseal|vasopressin-neurophysin II-copeptin
縮寫：

AVP
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, cell culture supernates
檢測范圍：

1.25 pg/mL-80 pg/mL
靈敏度：

0.31 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Neuroscience
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ADH/VP/AVP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-102
1:2	Average %	89
	Range %	84-95
1:4	Average %	87
	Range %	82-96
1:8	Average %	96
	Range %	90-104

回收率：

The recovery of mouse ADH/VP/AVP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	83-94
EDTA plasma (n=4)	102	98-107

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.