Mouse anti-thyroid-globulin antibody,TGAB ELISA Kit

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產(chǎn)品詳情

產(chǎn)品描述：

小鼠抗甲狀腺球蛋白抗體(ATGA/TGAB)酶聯(lián)免疫試劑盒（CSB-E09543m）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的TG Ab含量。TG Ab即甲狀腺球蛋白抗體，是自身免疫性甲狀腺疾病患者血清中常見的自身抗體。其背景與自身免疫紊亂相關(guān)。研究機(jī)制上，它可能通過破壞甲狀腺濾泡細(xì)胞、影響甲狀腺球蛋白代謝等，參與自身免疫性甲狀腺炎的發(fā)病過程，影響甲狀腺功能。試劑盒檢測范圍為1.25 U/mL-20 U/mL，適用于血清、血漿樣本的科研分析；在動物模型中常被用于研究甲狀腺自身免疫反應(yīng)的病理機(jī)制及藥物干預(yù)效果評估；適用于實驗室環(huán)境下的小鼠模型研究，包括甲狀腺自身免疫疾病發(fā)病機(jī)理探索、免疫調(diào)節(jié)藥物作用效果驗證以及相關(guān)生物標(biāo)志物動態(tài)監(jiān)測等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

TG Ab
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

1.25 U/mL-20 U/mL
靈敏度：

1.25 U/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse TGAB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-101
1:2	Average %	89
	Range %	85-98
1:4	Average %	92
	Range %	84-100
1:8	Average %	95
	Range %	90-100

回收率：

The recovery of mouse TGAB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-103
EDTA plasma (n=4)	95	90-105